Molecular Characterization and Identification of Target Protein of an Important Vesicle Trafficking GeneAlRab7from a Salt Excreting HalophyteAeluropus lagopoides
| Autor: | Jackson Khedia, Khantika Patel, Navya Rajan, Parinita Agarwal, Pradeep K. Agarwal, Payal Sanadhya |
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| Rok vydání: | 2015 |
| Předmět: |
DNA
Complementary Original Research ArticlesOrganelles/Autophagy/Apoptosis Aeluropus lagopoides Molecular Sequence Data ved/biology.organism_classification_rank.species Sodium Chloride Biology Poaceae Rab geranylgeranyltransferase Plant Growth Regulators Prenylation Gene Expression Regulation Plant Sequence Homology Nucleic Acid Two-Hybrid System Techniques Halophyte Genetics Endomembrane system Amino Acid Sequence Cloning Molecular Transport Vesicles Molecular Biology Phylogeny Plant Proteins Base Sequence Sequence Homology Amino Acid Reverse Transcriptase Polymerase Chain Reaction ved/biology fungi Water rab7 GTP-Binding Proteins Salt-Tolerant Plants Aeluropus Sequence Analysis DNA Cell Biology General Medicine biology.organism_classification Transport protein Biochemistry rab GTP-Binding Proteins Rab Carrier Proteins Abscisic Acid Protein Binding |
| Zdroj: | DNA and Cell Biology. 34:83-91 |
| ISSN: | 1557-7430 1044-5498 |
| Popis: | The endomembrane system plays an important role during cellular adaptation of the plants with the extracellular environment. The small GTP-binding protein Rab7 located at the vacuolar membrane regulates the vesicle fusion with the vacuole and thereby helps in recycling of the molecules. This is the first report on isolation and characterization of AlRab7 gene from the halophyte plant, Aeluropus that extrudes NaCl through salt glands and grows luxuriantly throughout the year at the Gujarat coast, India. The AlRab7 encodes a protein with 206 amino acids, and a highly conserved effector-binding domain and four nucleotide-binding domains. The in silico analysis predicts the presence of the prenylation site for Rab geranylgeranyltransferase 2 and the Rab escort protein site. The C-terminal two cysteine residues in –XCC sequence are present for membrane attachment. Transcript expression of the AlRab7 gene was differentially regulated by different environmental stimuli such as dehydration, salinity, and hormone abscisic acid (ABA). The recombinant Escherichia coli cells showed improved growth in Luria Bertani medium supplemented with NaCl, KCl, mannitol, ABA, and indole-3-acetic acid. A novel Rab7 interacting partner AlRabring7 was identified by yeast two-hybrid screening. |
| Databáze: | OpenAIRE |
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