Determination of Withanolides in Withania somnifera by Liquid Chromatography: Single-Laboratory Validation, First Action 2015.17
| Autor: | Rojison Koshy, Balasubramanian Murali, Sharon L Brunelle, Mayachari Shivanand Anand |
|---|---|
| Rok vydání: | 2017 |
| Předmět: |
Pharmacology
Analyte Chromatography biology 010405 organic chemistry 010401 analytical chemistry Extraction (chemistry) Withanoside IV Repeatability Withania somnifera biology.organism_classification 01 natural sciences 0104 chemical sciences Analytical Chemistry chemistry.chemical_compound Withanolide chemistry Withaferin A Performance requirement Environmental Chemistry Agronomy and Crop Science Food Science |
| Zdroj: | Journal of AOAC INTERNATIONAL. 100:277-279 |
| ISSN: | 1944-7922 1060-3271 |
| Popis: | An LC method was developed and validated in 2007 for analyzing Withania somnifera raw material (root) and dried extracts for withanolide content, including withanoside IV, withanoside V, withaferin A, 12-deoxywithastromonolide, withanolide A, and withanolide B. The method involved the extraction of the analytes with methanol, their subsequent filtration, and then analysis on a C18 column with an acetonitrile gradient and UV detection. Single-laboratory validation yielded linearity generally in the range of 20 to 200 μg/mL for each analyte, with a repeatability precision of RSD < 3% in most cases, and recovery in the range of 90 to 105%. These results compare well with the performance criteria recently detailed in AOAC Standard Method Performance Requirement 2015.007. The method was shown to be rugged with respect to different analysts, equipment, and days of analysis, and the sample solution was shown to be stable for 24 h at room temperature after extraction. The method was reviewed by the AOAC Expert Review Panel on Dietary Supplements (Set 2 Ingredients) and approved for First Action Official MethodSM status. |
| Databáze: | OpenAIRE |
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