Ascorbic Acid Attenuates Multifidus Muscles Injury and Atrophy After Posterior Lumbar Spine Surgery by Suppressing Inflammation and Oxidative Stress in a Rat Model
| Autor: | Ren Zhu, Jia-Qi Xu, Jiying Wang, Pan Tang, Shunwu Fan, Ziang Xie, Yu Gu, Kang-Mao Huang, Zhi-Jun Hu, Xue-Sheng Jiang, Xiangde Zhao, Jia-Ming Gu |
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| Rok vydání: | 2018 |
| Předmět: |
Male
0301 basic medicine medicine.medical_specialty Paraspinal Muscles Urology Inflammation Ascorbic Acid medicine.disease_cause Antioxidants Neurosurgical Procedures Multifidus muscle 03 medical and health sciences chemistry.chemical_compound 0302 clinical medicine Atrophy Fibrosis Malondialdehyde Animals Medicine Orthopedic Procedures Orthopedics and Sports Medicine Lumbar Vertebrae Interleukin-6 Superoxide Dismutase Tumor Necrosis Factor-alpha business.industry Sham surgery medicine.disease Ascorbic acid Rats Muscular Atrophy Oxidative Stress 030104 developmental biology Adipose Tissue chemistry Neurology (clinical) medicine.symptom business 030217 neurology & neurosurgery Oxidative stress |
| Zdroj: | Spine. 43:E1249-E1259 |
| ISSN: | 1528-1159 0362-2436 |
| DOI: | 10.1097/brs.0000000000002669 |
| Popis: | Study design A rat model of multifidus muscles injury and atrophy after posterior lumbar spine surgery. Objective We determined the effect of ascorbic acid (AA) on the postoperative multifidus muscles in rat model. Summary of background data Previous studies show oxidative stress and inflammation are two main molecular mechanisms in multifidus muscle injury and atrophy after posterior lumbar surgery. AA may have a protective effect in postoperative multifidus muscles. Methods Rats were divided into sham surgery, control surgery, and surgery plus AA groups. Multifidus muscles of the control and AA groups were excised from the osseous structures. The muscles were retracted continuously for 2 hours. In the sham and AA groups, AA was administered via oral gavage daily in the first week. In each group, the oxidative stress was evaluated by measuring malondialdehyde (MDA) and Total superoxide dismutase (T-SOD). The inflammation, fat degeneration, or fibrosis of multifidus muscle were evaluated by quantitative real-time polymerase chain reaction (q-PCR), histology, or immunohistochemical analysis. Results T-SOD activity was significantly lower in the control group than that in the AA group in the first week. MDA levels were significantly higher in the AA group. Interleukin-6 and tumor necrosis factor-α in multifidus muscles also showed significant differences when treated with AA. The inflammation score on histology was significantly lower in the AA group postoperatively in the first week. In the long run, marker genes for fibrosis and fat degeneration, and fibrosis and fat degeneration scores, were significantly lower in the AA than the control group on days 14 and 28 postoperatively. Conclusion In conclusion, AA attenuated the oxidative stress and inflammation response in the postoperative multifidus muscles, and remarkable differences were observed from the histological assessment and related marker genes expression. Our results provided important insight into the anti-inflammatory and anti-oxidative effects of AA in the postoperative multifidus muscles. Level of evidence N/A. |
| Databáze: | OpenAIRE |
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