Variable sources of Bk virus in renal allograft recipients
| Autor: | Laura Masami Sumita, Cláudio Sérgio Pannuti, Lígia Camera Pierrotti, Elias David-Neto, Camila Malta Romano, Paulo Roberto Palma Urbano, Luiz Henrique da Silva Nali, Renato Reis Oliveira, Camila da Silva Bicalho, Maria Cristina Domingues da Silva Fink |
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| Rok vydání: | 2018 |
| Předmět: |
Adult
Male Human polyomavirus BK Viremia Biology medicine.disease_cause Kidney Nephropathy 03 medical and health sciences 0302 clinical medicine Virology medicine Humans Transplantation Homologous 030212 general & internal medicine Genetic variability Prospective Studies Phylogeny Aged Polyomavirus Infections Genetic Variation Middle Aged medicine.disease Kidney Transplantation Transplant Recipients BK virus Transplantation Tumor Virus Infections Infectious Diseases Real-time polymerase chain reaction medicine.anatomical_structure BK Virus DNA Viral 030211 gastroenterology & hepatology Female Kidney Diseases |
| Zdroj: | Journal of medical virology. 91(6) |
| ISSN: | 1096-9071 |
| Popis: | BK virus is the causative agent of polyomavirus-associated nephropathy, a major cause of kidney transplant failure affecting 1%-10% of recipients. Previous studies that investigated the viral source on the kidney recipient pointed that the donor is implicated in the origin of human polyomavirus BK (BKPyV) infection in recipients, but giving the low genetic variability of BKPyV this subject is still controversial. The aim of this study was to determine if BKPyV replicating in kidney recipients after transplantation is always originated from the donor. Urine and blood samples from 68 pairs of living donors and kidney recipients who underwent renal transplantation from August 2010-September 2011 were screened for BKPyV by real time polymerase chain reaction. Only three recipients presented viremia. When both donors and recipients were BKPyV positive, a larger fragment of VP1 region was obtained and sequenced to determine the level of similarity between them. A phylogenetic tree was built for the 12 pairs of sequences obtained from urine and high level of similarity among all sequences was observed, indicating that homology inferences for donor and recipient viruses must be cautiously interpreted. However, a close inspection on the donor-recipient pairs sequences revealed that 3 of 12 pairs presented considerably different viruses and 4 of 12 presented mixed infection, indicating that the source of BKPyV infection is not exclusively derived from the donor. We report that about 60% of the renal recipients shed BKPyV genetically distinct from the donor, confronting the accepted concept that the donor is the main source of recipients' infection. |
| Databáze: | OpenAIRE |
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