The binding of bridged bis-pyridinium oximes to DNA and its relevance to the induction of mitochondrial dysfunction in yeast
| Autor: | Sylvano Nocentini, Pierre Demerseman, Guy Dodin, Jean-Marc Kühnel, Dietrich Averbeck |
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| Rok vydání: | 1992 |
| Předmět: |
Stereochemistry
Pyridines Saccharomyces cerevisiae Biophysics Mitochondrion medicine.disease_cause Nucleic Acid Denaturation Biochemistry chemistry.chemical_compound Structure-Activity Relationship Polydeoxyribonucleotides Oximes medicine Animals Binding site DNA Fungal Molecular Biology Organelle inner membrane Mutation Binding Sites biology Cell Biology DNA biology.organism_classification Oxime Binding constant Intercalating Agents Mitochondria Kinetics chemistry Cattle Plasmids |
| Zdroj: | Biochemical and biophysical research communications. 186(3) |
| ISSN: | 0006-291X |
| Popis: | Summary Bis-pyridium oximes and methoximes from a newly synthesized series are weak DNA binders (K= 3.104 M−1 under physiological conditions). From the number of binding sites per phosphate, 0.25, the ionic strength dependence of the binding constant and the negative electric dichroism, it is concluded that monointercalation is the mode of association. In contrast to methoxy compounds, the oxime derivatives are able both to induce the mutated “petite” phenotype in yeast S. cerivisiae and to cause “in vitro” extensive condensation of single stranded DNA. This reaction is postulated to be relevant to the mutational process that leads to “petite” cells. The absence of nuclear mutation is interpreted in terms of sequestration of the drug in mitochondria under the effect of the organelle inner membrane electrochemical potential. |
| Databáze: | OpenAIRE |
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