Spike-timing relationship of neurochemically-identified dorsal raphe neurons during cortical slow oscillations

Autor: Nicolas Mallet, Trevor Sharp, Jv V. Schweimer, Mark A. Ungless
Přispěvatelé: University of St Andrews. School of Psychology and Neuroscience
Rok vydání: 2011
Předmět:
Male
Cognitive
Behavioral
and Systems Neuroscience
Time Factors
Dopamine
5-HT
Action Potentials
PBS-X
PBS containing 0.2% Triton X-100
ANOVA
analyses of variance
Rats
Sprague-Dawley
PFC
prefrontal cortex
Limbic system
limbic system
Basal ganglia
Cerebral Cortex
5-HT
5-hydroxytryptamine
General Neuroscience
ECoG
electrocorticogram
Electroencephalography
COV-IS
coefficient of variation of the inter-spike-interval
Immunohistochemistry
medicine.anatomical_structure
SWA
slow-wave activity
basal ganglia
dopamine
RC0321 Neuroscience. Biological psychiatry. Neuropsychiatry
Research Paper
Serotonergic Neurons
TH
tyrosine hydroxylase
Neuroscience(all)
PBS
phosphate-buffered saline
serotonergic
Biology
Serotonergic
Bursting
Dorsal raphe nucleus
Neurochemical
DRN
dorsal raphe nucleus
medicine
Animals
Brain Waves
Rats
Electrophysiology
nervous system
RC0321
Raphe Nuclei
Serotonin
Neuroscience
Zdroj: Neuroscience
ISSN: 0306-4522
DOI: 10.1016/j.neuroscience.2011.08.072
Popis: The firing activity of dorsal raphe neurons is related to arousal state. However, it is unclear how this firing activity is precisely related to cortical activity, in particular oscillations occurring during sleep rhythms. Here we conducted single-cell extracellular recordings and juxtacellular labelling while monitoring electrocorticogram (ECoG) activity in urethane anaesthetised rats, to relate activity in neurochemically identified groups of neurons to cortical slow-wave activity (SWA). We observed that electrophysiological heterogeneity in dorsal raphe neurons revealed different neurochemical groups of DRN neurons and was mirrored by significant differences in the phase and strength of coupling to the cortical slow oscillations. Spike firing relationship of clock-like neurons, identified as 5-HT (5-hydroxytryptamine) or serotonin neurons, was higher during the inactive component of the oscillations. In contrast, half of the identified bursting 5-HT neurons did not exhibit strong cortical entrainment; those that did fired most during the inactive component of the SWA. Two groups of putatively non-5-HT neurons (irregular slow-firing and fast-firing) exhibited significant coherence and fired most during the active component of the SWA. These findings indicate that within the DRN electrophysiologically and neurochemically discrete neuronal groups exhibit distinct relations to cortical activity.
Highlights ▶DRN neurons exhibit heterogeneous firing in relation to cortical oscillations. ▶Clock-like 5-HT neurons fire most during inactive component of the oscillation. ▶Half of bursting 5-HT neurons did not exhibit coupling to the oscillation. ▶Non-5-HT neurons fired most during the active component of the oscillation.
Databáze: OpenAIRE
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