In Vitro Activity of a Novel Glycopolymer against Biofilms of Burkholderia cepacia Complex Cystic Fibrosis Clinical Isolates
| Autor: | Vidya P. Narayanaswamy, Andrew P. Duncan, Stacy M. Townsend, William P. Wiesmann, John J. LiPuma, Shenda M. Baker |
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| Rok vydání: | 2019 |
| Předmět: |
Burkholderia gladioli
Burkholderia cenocepacia Burkholderia antimicrobial agents confocal microscopy Microbiology 03 medical and health sciences glycopolymer Mechanisms of Resistance PAAG Burkholderia dolosa Pharmacology (medical) skin and connective tissue diseases 030304 developmental biology Pharmacology 0303 health sciences antimicrobial activity integumentary system biology 030306 microbiology Chemistry Burkholderia multivorans Biofilm biochemical phenomena metabolism and nutrition biology.organism_classification respiratory tract diseases Burkholderia cepacia complex Infectious Diseases Burkholderia vietnamiensis biofilms |
| Zdroj: | Antimicrobial Agents and Chemotherapy |
| ISSN: | 1098-6596 0066-4804 |
| DOI: | 10.1128/aac.00498-19 |
| Popis: | Burkholderia cepacia complex (Bcc) lung infections in cystic fibrosis (CF) patients are often associated with a steady decline in lung function and death. The formation of biofilms and inherent multidrug resistance are virulence factors associated with Bcc infection and contribute to increased risk of mortality in CF patients. Burkholderia cepacia complex (Bcc) lung infections in cystic fibrosis (CF) patients are often associated with a steady decline in lung function and death. The formation of biofilms and inherent multidrug resistance are virulence factors associated with Bcc infection and contribute to increased risk of mortality in CF patients. New therapeutic strategies targeting bacterial biofilms are anticipated to enhance antibiotic penetration and facilitate resolution of infection. Poly (acetyl, arginyl) glucosamine (PAAG) is a cationic glycopolymer therapeutic being developed to directly target biofilm integrity. In this study, 13 isolates from 7 species were examined, including Burkholderia multivorans, Burkholderia cenocepacia, Burkholderia gladioli, Burkholderia dolosa, Burkholderia vietnamiensis, and B. cepacia. These isolates were selected for their resistance to standard clinical antibiotics and their ability to form biofilms in vitro. Biofilm biomass was quantitated using static tissue culture plate (TCP) biofilm methods and a minimum biofilm eradication concentration (MBEC) assay. Confocal laser scanning microscopy (CLSM) visualized biofilm removal by PAAG during treatment. Both TCP and MBEC methods demonstrated a significant dose-dependent relationship with regard to biofilm removal by 50 to 200 μg/ml PAAG following a 1-h treatment (P |
| Databáze: | OpenAIRE |
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