Design of a Seed-Specific Chimeric Promoter with a Modified Expression Profile to Improve Seed Oil Content

Autor: Akiko Kozaki, Masaya Kobayashi, Toshihiro Aoyagi
Rok vydání: 2018
Předmět:
0106 biological sciences
0301 basic medicine
Arabidopsis
Genetically modified crops
01 natural sciences
lcsh:Chemistry
Gene Expression Regulation
Plant
Gene expression
WRINKLED 1
biotin carboxyl carrier protein 2
Promoter Regions
Genetic
lcsh:QH301-705.5
chimeric promoter
Spectroscopy
diacylglycerol acyltransferase 1
biology
Fatty Acids
Seed Storage Proteins
food and beverages
General Medicine
Plants
Genetically Modified
Computer Science Applications
Cell biology
seed oil
Seeds
Fatty Acid Elongases
Protein subunit
Biotin carboxyl carrier protein
fatty acid elongase 1
Article
Catalysis
Inorganic Chemistry
03 medical and health sciences
Acetyltransferases
Plant Oils
Diacylglycerol O-Acyltransferase
Physical and Theoretical Chemistry
Molecular Biology
Gene
Arabidopsis Proteins
Organic Chemistry
Acetyl-CoA carboxylase
Promoter
biology.organism_classification
triacyl glycerol
030104 developmental biology
lcsh:Biology (General)
lcsh:QD1-999
biology.protein
Acetyl-CoA Carboxylase
010606 plant biology & botany
Zdroj: International Journal of Molecular Sciences
Volume 19
Issue 6
International Journal of Molecular Sciences, Vol 19, Iss 6, p 1667 (2018)
ISSN: 1422-0067
DOI: 10.3390/ijms19061667
Popis: Increasing the yield of plant oil is an important objective to meet the demand for sustainable resources and energy. Some attempts to enhance the expression of genes involved in oil synthesis in seeds have succeeded in increasing oil content. In many cases, the promoters of seed-storage protein genes have been used as seed-specific promoters. However, conventional promoters are developmentally regulated and their expression periods are limited. We constructed a chimeric promoter that starts to express in the early stage of seed development, and high-level expression is retained until the later stage by connecting the promoters of the biotin carboxyl carrier protein 2 (BCCP2) gene encoding the BCCP2 subunit of acetyl-CoA carboxylase and the fatty acid elongase 1 (FAE1) gene from Arabidopsis. The constructed promoter was ligated upstream of the TAG1 gene encoding diacylglycerol acyltransferase 1 and introduced into Arabidopsis. Seeds from transgenic plants carrying AtTAG1 under the control of the chimeric promoter showed increased oil content (up by 18&ndash
73%) compared with wild-type seeds. The novel expression profile of the chimeric promoter showed that this could be a promising strategy to manipulate the content of seed-storage oils and other compounds.
Databáze: OpenAIRE
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