Intestinal Lactobacillus sp. is associated with some cellular and metabolic characteristics of blood in elderly people
| Autor: | Jelena Štšepetova, Mihkel Zilmer, Kersti Zilmer, Pirje Hütt, Krista Lõivukene, Helgi Kolk, Epp Sepp, Marika Mikelsaar |
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| Rok vydání: | 2010 |
| Předmět: |
Blood Glucose
Estonia Male Lactobacillus casei Lactobacillus paracasei Lactobacillus fermentum Physiology Blood sugar Pilot Projects Microbiology Leukocyte Count Lactobacillus acidophilus Species Specificity medicine Humans Aged Inflammation biology Probiotics food and beverages biology.organism_classification medicine.disease Lactobacillus reuteri Intestines Lipoproteins LDL Lactobacillus Infectious Diseases Immunology Female Metabolic syndrome Lactobacillus plantarum |
| Zdroj: | Anaerobe. 16:240-246 |
| ISSN: | 1075-9964 |
| DOI: | 10.1016/j.anaerobe.2010.03.001 |
| Popis: | The higher counts or particular groups (Firmicutes/Bacteroidetes) of intestinal microbiota are related to host metabolic reactions, supporting a balance of human ecosystem. We further explored whether intestinal lactobacilli were associated with some principal cellular and metabolic markers of blood in 38 healthy >65-year-old persons. The questionnaire, routine clinical and laboratory data of blood indices as much as the oxidized low-density lipoprotein (ox-LDL) and baseline diene conjugates in low-density lipoprotein (BDC-LDL) of blood sera were explored. The PCR-based intestinal Lactobacillus sp. composition and counts of cultivable lactobacilli (LAB) were tested. The facultative heterofermentative lactobacilli (Lactobacillus casei and Lactobacillus paracasei) were the most frequent (89 and 97%, respectively) species found, while Lactobacillus acidophilus, Lactobacillus plantarum and Lactobacillus reuteri were present in almost half of the elderly persons. The number of species simultaneously colonizing the individuals ranged from 1 to 7 (median 4). In elderly consuming probiotics the LAB counts were significantly higher than in these not consuming (median 7.8, range 4.2-10.8 vs. median 6.3, range 3.3-9.7 log cfu/g; p=0.005), adjusted (OR=1.71, CI95 1.04-2.82; p=0.035) for age and body mass index (BMI). The colonization by L. acidophilus was negatively related (r=-0.367, p=0.0275) to L. reuteri, staying significant after adjusting for age, sex and BMI (OR=0.16, CI95 0.04-0.73; p=0.018). However, the blood glucose concentration showed a tendency for a negative correlation for colonization with Lactobacillus fermentum (r=-0.309, p=0.062) adjusted for BMI (Adj. R(2)=0.181; p=0.013) but not for age and sex. The higher white blood cells (WBC) count was positively related (r=0.434, p=0.007) to presence of Lactobacillus reuteri adjusted for age, sex and BMI (Adj. R(2)=0.193, p=0.027). The lower values of ox-LDL were predicted by higher counts of cultivable lactobacilli adjusted by sex, age and BMI (r = -0.389, p = 0.016; Adj. R(2)=0.184 p=0.029). In conclusion, the pilot study of elderly persons shows that the intestinal lactobacilli are tightly associated with WBC count, blood glucose and content of ox-LDL which all serve as risk markers in pathogenesis of inflammation, metabolic syndrome and cardiovascular disease (CVD). |
| Databáze: | OpenAIRE |
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