Species diversity, antifungal susceptibility and phenotypic and genotypic characterisation of Exophiala spp. infecting patients in different medical centres in Brazil
| Autor: | Daniel Wagner de Castro Lima Santos, Sarah Santos Gonçalves, Analy Salles de Azevedo Melo, Ana Carolina Barbosa Padovan, Wendy C. Silva, Fernando César Bizerra |
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| Rok vydání: | 2016 |
| Předmět: |
0301 basic medicine
Adult Male Posaconazole Antifungal Agents Adolescent Genotype Itraconazole 030106 microbiology Dermatology Microbial Sensitivity Tests Biology Microbiology 03 medical and health sciences chemistry.chemical_compound Echinocandins Lipopeptides 0302 clinical medicine Exophiala Caspofungin Amphotericin B DNA Ribosomal Spacer medicine Humans 030212 general & internal medicine Child Aged Voriconazole Genetic Variation General Medicine Middle Aged biology.organism_classification medicine.disease Phaeohyphomycosis Infectious Diseases Phenotype chemistry Child Preschool Microscopy Electron Scanning Terbinafine Female Brazil medicine.drug |
| Zdroj: | Mycoses. 60(5) |
| ISSN: | 1439-0507 |
| Popis: | The Exophiala genus is responsible for many superficial and invasive infections resulting from black fungi. Identification of Exophiala at the species level is based on morphological observations complemented by molecular tests. The aim of this study was to identify 23 clinical isolates of Exophiala spp. and evaluate the antifungal susceptibility to seven different agents. Molecular identification was based on an analysis of ITS region of rDNA using genomic databases. The micromorphology was evaluated by microculture and scanning electron microscopy. The susceptibility tests were performed using the antifungal agents 5-fluorocytosine (5-FC), amphotericin B (AMB), itraconazole (ITC), voriconazole (VRC), posaconazole (PSC), caspofungin (CFG) and terbinafine (TRB). The ITS analysis identified 100% of the following isolates as: E. dermatitidis (8), E. xenobiotica (6), E. bergeri (4), E. oligosperma (3), E. spinifera (1) and E. mesophila (1). The antifungal susceptibility tests showed that the triazoles compounds were in vitro the most active agents against Exophiala. ITS sequencing enabled the accurate identification of the 23 tested isolates. The triazoles, particularly itraconazole and posaconazole, exhibited MIC values lower than AMB, CAS and 5-FC. Although the guidelines do not indicate AMB for treatment against Exophiala spp., this study showed activity for all of the tested species, except E. mesophila. |
| Databáze: | OpenAIRE |
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