Proliferación y diferenciación osteogénica de células madre mesenquimales en hidrogeles de plasma sanguíneo humano
| Autor: | Adriana Doncel, Itali Linero, Orlando Chaparro |
|---|---|
| Rok vydání: | 2013 |
| Předmět: |
medicine.medical_specialty
lcsh:Arctic medicine. Tropical medicine stem cells hydrogels plasma bone regeneration lcsh:RC955-962 Regeneration (biology) lcsh:R Mesenchymal stem cell lcsh:Medicine Adipose tissue Biology Regenerative medicine General Biochemistry Genetics and Molecular Biology Surgery Cell biology Cell therapy medicine Bone regeneration Multipotentiality Explant culture |
| Zdroj: | Biomédica: revista del Instituto Nacional de Salud, Vol 34, Iss 1, Pp 67-78 (2014) |
| ISSN: | 0120-4157 |
| DOI: | 10.7705/biomedica.v34i1.1465 |
| Popis: | Introduction: The use of mesenchymal stem cells in clinical practice has increased considerably in the last decade because they play a supporting role in the processes of tissue repair and regeneration, becoming the main tool of cell therapy for the treatment of diseases functionally affecting bone and cartilage tissue. Objective: To evaluate in vitro the proliferative and osteogenic differentiation ability of mesenchymal stem cells derived from human adipose tissue in a blood plasma hydrogel. Materials and methods: Mesenchymal stem cells were obtained from human adipose tissue explants and characterized by flow cytometry. Their multipotentiality was demonstrated by their ability to differentiate to adipogenic and osteogenic lineages. Cell proliferation and osteogenic differentiation ability of the cells cultured in blood plasma hydrogels were also evaluated. Results: Mesenchymal stem cells derived from human adipose tissue growing in human blood plasma hydrogels showed a pattern of proliferation similar to that of the cells cultured in monolayer and also maintained their ability to differentiate to osteogenic lineage. Conclusions: Human blood plasma hydrogels are a suitable support for proliferation and osteogenic differentiation of mesenchymal stem cells derived from human adipose tissue and provides a substrate that is autologous, biocompatible, reabsorbable, easy to use, potentially injectable and economic, which could be used as a successful strategy for the management and clinical application of cell therapy in regenerative medicine. |
| Databáze: | OpenAIRE |
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