A comparative study of Lonicera japonica with related species: Morphological characteristics, ITS sequences and active compounds
| Autor: | Yang Yu, Shan Li, Qing-Xia Wang, Yunguo Zhu, Yunlong Lan, Xueli Zhou, Zhou Cheng, Qing-Mei Quan |
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| Rok vydání: | 2014 |
| Předmět: |
Cfr I
Identification biology Lonicera japonica Lonicera confusa Flos Lonicera fulvotomentosa biology.organism_classification Flos Lonicerae (FL) Biochemistry Article Japonica Lonicera macranthoides chemistry.chemical_compound Chlorogenic acid chemistry Botany Flos Lonicerae Japonicae (FLJ) Ecology Evolution Behavior and Systematics Lonicera hypoglauca |
| Zdroj: | Biochemical Systematics and Ecology |
| ISSN: | 0305-1978 |
| DOI: | 10.1016/j.bse.2014.02.002 |
| Popis: | Morphological characteristics, ITS sequences, and active compounds have been used to differentiate between species of Lonicera used in the traditional Chinese medicines Flos Lonicerae Japonicae (FLJ) and Flos Lonicerae (FL). FLJ includes L. japonica whereas FJ includes Lonicera macranthoides, Lonicera hypoglauca, Lonicera confusa and Lonicera fulvotomentosa. FLJ could be distinguished from FL using four quantitative and 10 qualitative characters, ITS sequences, chlorogenic acid and luteoloside contents. Analyses revealed that L. japonica was very different from the other species. The results have implications for the identification and quality control of species of Lonicera used medicinally, suggesting that species should not be interchanged in medicinal preparations. Highlights • Morphological characteristics, ITS sequences and active compounds of 30 Lonicera germplasm were compared. • Flos Lonicerae Japonicae (FLJ) and Flos Lonicerae (FJ) could be distinguished. • FLJs are easy to identify from FL by Cfr I digested reaction of the ITS PCR products. |
| Databáze: | OpenAIRE |
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