Cooperation between myogenic regulatory factors and SIX family transcription factors is important for myoblast differentiation
| Autor: | Alphonse Chu, Alexandre Blais, Yubing Liu, Uzma Islam, Imane Chakroun |
|---|---|
| Rok vydání: | 2010 |
| Předmět: |
Transcriptional Activation
Myoblasts Skeletal Cellular differentiation Gene Regulation Chromatin and Epigenetics Biology Muscle Development Cell Line Mice 03 medical and health sciences 0302 clinical medicine Gene expression Genetics Animals Humans Myocyte Transcription factor 030304 developmental biology Homeodomain Proteins Regulation of gene expression 0303 health sciences Genome Myogenesis Cell Differentiation Cell biology Myogenic Regulatory Factors Regulatory sequence Myogenic regulatory factors Trans-Activators 030217 neurology & neurosurgery |
| Zdroj: | Nucleic Acids Research |
| ISSN: | 1362-4962 0305-1048 |
| DOI: | 10.1093/nar/gkq585 |
| Popis: | Precise regulation of gene expression is crucial to myogenesis and is thought to require the cooperation of various transcription factors. On the basis of a bioinformatic analysis of gene regulatory sequences, we hypothesized that myogenic regulatory factors (MRFs), key regulators of skeletal myogenesis, cooperate with members of the SIX family of transcription factors, known to play important roles during embryonic skeletal myogenesis. To this day little is known regarding the exact molecular mechanism by which SIX factors regulate muscle development. We have conducted a functional genomic study of the role played by SIX1 and SIX4 during the differentiation of skeletal myoblasts, a model of adult muscle regeneration. We report that SIX factors cooperate with the members of the MRF family to activate gene expression during myogenic differentiation, and that their function is essential to this process. Our findings also support a model where SIX factors function not only ‘upstream’ of the MRFs during embryogenesis, but also ‘in parallel’ to them during myoblast differentiation. We have identified new essential nodes that depend on SIX factor function, in the myogenesis regulatory network, and have uncovered a novel way by which MRF function is modulated during differentiation. |
| Databáze: | OpenAIRE |
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