Arl2- and Msps-dependent microtubule growth governs asymmetric division
| Autor: | Chwee Tat Koe, Wenhui Zong, Quan Tang, Xiaolin Tian, Sharyn A. Endow, Chunlai Wu, Wanjin Hong, Fengwei Yu, Hongyan Wang, Fabrizio Rossi, Keng Chen, Cheng Wang, Cayetano Gonzalez, Zhanyuan Benny Xing, Reshma Taneja |
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| Rok vydání: | 2016 |
| Předmět: |
0301 basic medicine
animal structures Cell division Microtubule-associated protein Dynein Microtubules Article 03 medical and health sciences Neural Stem Cells Tubulin Microtubule Cell polarity Animals Drosophila Proteins Research Articles Cells Cultured Centrosome Genetics biology ADP-Ribosylation Factors fungi Cell Polarity Membrane Proteins Cell Biology Neural stem cell Cell biology Drosophila melanogaster 030104 developmental biology nervous system biology.protein Microtubule-Associated Proteins Cell Division Protein Binding |
| Zdroj: | The Journal of Cell Biology |
| ISSN: | 1540-8140 0021-9525 |
| Popis: | Drosophila Arl2 governs neuroblast asymmetric cell division through regulation of microtubule growth and localization of Msps to centrosomes. Asymmetric division of neural stem cells is a fundamental strategy to balance their self-renewal and differentiation. It is long thought that microtubules are not essential for cell polarity in asymmetrically dividing Drosophila melanogaster neuroblasts (NBs; neural stem cells). Here, we show that Drosophila ADP ribosylation factor like-2 (Arl2) and Msps, a known microtubule-binding protein, control cell polarity and spindle orientation of NBs. Upon arl2 RNA intereference, Arl2-GDP expression, or arl2 deletions, microtubule abnormalities and asymmetric division defects were observed. Conversely, overactivation of Arl2 leads to microtubule overgrowth and depletion of NBs. Arl2 regulates microtubule growth and asymmetric division through localizing Msps to the centrosomes in NBs. Moreover, Arl2 regulates dynein function and in turn centrosomal localization of D-TACC and Msps. Arl2 physically associates with tubulin cofactors C, D, and E. Arl2 functions together with tubulin-binding cofactor D to control microtubule growth, Msps localization, and NB self-renewal. Therefore, Arl2- and Msps-dependent microtubule growth is a new paradigm regulating asymmetric division of neural stem cells. |
| Databáze: | OpenAIRE |
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