Spatio-temporal localization of HIF-1α and COX-2 during irradiation-induced oral mucositis in a rat model system

Autor:	Hong-Wei Jiang, Chun-Yang Li, Jun-Bing Guo, Chong-Jin Feng, Bin Cheng, Shuang-Xi Zhu, Han-Yu Wang, Yong Chen
Rok vydání:	2008
Předmět:	Pathology medicine.medical_specialty medicine.medical_treatment Rat model Rats Sprague-Dawley Gene expression medicine Mucositis Animals Radiology Nuclear Medicine and imaging RNA Messenger Irradiation Stomatitis Radiological and Ultrasound Technology Reverse Transcriptase Polymerase Chain Reaction Chemistry Head and neck cancer Mouth Mucosa Hypoxia (medical) Hypoxia-Inducible Factor 1 alpha Subunit medicine.disease Rats Radiation therapy Radiation Injuries Experimental Cyclooxygenase 2 medicine.symptom
Zdroj:	International Journal of Radiation Biology. 84:35-45
ISSN:	1362-3095 0955-3002
DOI:	10.1080/09553000701616080
Popis:	Oral mucositis is a common side effect of radiotherapy for head and neck cancer. The purpose of this study was to examine the significance of and the relationship between hypoxia inducible factor-1alpha (HIF-1alpha) and cyclooxygenase-2 (COX-2) gene expression and the corresponding protein levels in irradiated rat mucosa.A Sprague-Dawley rat model of irradiation-induced oral mucositis was generated. Semi-quantitative reverse transcription-polymerase chain reaction (RT-PCR) was used to evaluate the HIF-1alpha and COX-2 mRNA level in rat buccal mucosa exposed to a fractionated irradiation regime. The Streptavidin-Biotin-Complex method was applied to delineate the in situ localization, intensity, and distribution of both proteins. The right buccal mucosa was not irradiated and used as control tissue.The RT-PCR analyses demonstrated that, upon irradiation, HIF-1alpha and COX-2 expression was significantly induced in the left buccal mucosa in contrast to control buccal mucosa. Based on immunohistochemical analyses, the HIF-1alpha and COX-2 level, in situ localization, and the type of cells exhibiting the highest HIF-1alpha and COX-2 amounts appear to correlate.The expression and protein levels of HIF-1alpha and COX-2 are substantially enhanced in irradiated rat mucosa and correlate with each other and with the severity of irradiation-induced oral mucositis.
Databáze:	OpenAIRE
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