MicroRNA Expression Profiling in Psoriatic Arthritis

Autor: Giuseppe Argentino, Piera Filomena Fiore, Marzia Dolcino, Giuseppe Patuzzo, Andrea Pelosi, Antonio Puccetti, Francesca Moretta, Elisa Tinazzi, Claudio Lunardi
Rok vydání: 2018
Předmět:
Adult
Male
Genetics and Molecular Biology (all)
0301 basic medicine
Article Subject
Immunology and Microbiology (all)
Inflammatory arthritis
lcsh:Medicine
Arthritis
Arthritis
Psoriatic
Biomarkers
Female
Gene Expression Profiling
Humans
Jurkat Cells
MicroRNAs
Middle Aged
Signal Transduction
Transcriptome
Psoriatic
Biology
urologic and male genital diseases
Biochemistry
General Biochemistry
Genetics and Molecular Biology
03 medical and health sciences
Psoriatic arthritis
0302 clinical medicine
microRNA
medicine
Epigenetics
General Immunology and Microbiology
lcsh:R
Wnt signaling pathway
General Medicine
medicine.disease
Gene expression profiling
030104 developmental biology
Biochemistry
Genetics and Molecular Biology (all)
Immunology and Microbiology (all)
030220 oncology & carcinogenesis
Cancer research
Research Article
Zdroj: BioMed Research International, Vol 2018 (2018)
BioMed Research International
ISSN: 2314-6141
2314-6133
DOI: 10.1155/2018/7305380
Popis: Background. Psoriatic arthritis (PsA) is an inflammatory arthritis, characterized by bone erosions and new bone formation. MicroRNAs (miRNAs) are key regulators of the immune responses. Differential expression of miRNAs has been reported in several inflammatory autoimmune diseases; however, their role in PsA is not fully elucidated. We aimed to identify miRNA expression signatures associated with PsA and to investigate their potential implication in the disease pathogenesis. Methods. miRNA microarray was performed in blood cells of PsA patients and healthy controls. miRNA pathway analyses were performed and the global miRNA profiling was combined with transcriptome data in PsA. Deregulation of selected miRNAs was validated by real-time PCR. Results. We identified specific miRNA signatures associated with PsA patients with active disease. These miRNAs target pathways relevant in PsA, such as TNF, MAPK, and WNT signaling cascades. Network analysis revealed several miRNAs regulating highly connected genes within the PsA transcriptome. miR-126-3p was the most downregulated miRNA in active patients. Noteworthy, miR-126 overexpression induced a decreased expression of genes implicated in PsA. Conclusions. This study sheds light on some epigenetic aspects of PsA identifying specific miRNAs, which may represent promising candidates as biomarkers and/or for the design of novel therapeutic strategies in PsA.
Databáze: OpenAIRE
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