A pseudotype baculovirus expressing the capsid protein of foot-and-mouth disease virus and a T-Cell immunogen shows enhanced immunogenicity in mice
| Autor: | Pu Sun, Xiangtao Liu, Zaixin Liu, Yuanfang Fu, Zengjun Lu, Yimei Cao, Xiaofang Hao, Huifang Bao, Feipeng Tian |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2011 |
| Předmět: |
Immunogen
T-Lymphocytes viruses Genetic Vectors Antibodies Viral Injections Intramuscular Epitope lcsh:Infectious and parasitic diseases Interferon-gamma Mice Virology Animals lcsh:RC109-216 Neutralizing antibody Drug Carriers biology Research Immunogenicity Viral Vaccine Viral Vaccines biology.organism_classification Antibodies Neutralizing Titer Infectious Diseases Foot-and-Mouth Disease Virus Foot-and-Mouth Disease Inactivated vaccine biology.protein Capsid Proteins Foot-and-mouth disease virus Baculoviridae |
| Zdroj: | Virology Journal, Vol 8, Iss 1, p 77 (2011) Virology Journal |
| Popis: | Background Foot-and-mouth disease (FMD) is a highly contagious disease of livestock which causes severe economic loss in cloven-hoofed animals. Vaccination is still a major strategy in developing countries to control FMD. Currently, inactivated vaccine of FMDV has been used in many countries with limited success and safety concerns. Development of a novel effective vaccine is must. Methods In the present study, two recombinant pseudotype baculoviruses, one expressing the capsid of foot-and-mouth disease virus (FMDV) under the control of a cytomegalovirus immediate early enhancer/promoter (CMV-IE), and the other the caspid plus a T-cell immunogen coding region under a CAG promoter were constructed, and their expression was characterized in mammalian cells. In addition, their immunogenicity in a mouse model was investigated. The humoral and cell-mediated immune responses induced by pseudotype baculovirus were compared with those of inactivated vaccine. Results Indirect immunofluorescence assay (IFA) and indirect sandwich-ELISA (IS-ELISA) showed both recombinant baculoviruses (with or without T-cell epitopes) were transduced efficiently and expressed target proteins in BHK-21 cells. In mice, intramuscular inoculation of recombinants with 1 × 109 or 1 × 1010 PFU/mouse induced the production of FMDV-specific neutralizing antibodies and gamma interferon (IFN-γ). Furthermore, recombinant baculovirus with T-cell epitopes had better immunogenicity than the recombinant without T-cell epitopes as demonstrated by significantly enhanced IFN-γ production (P < 0.01) and higher neutralizing antibody titer (P < 0.05). Although the inactivated vaccine produced the highest titer of neutralizing antibodies, a lower IFN-γ expression was observed compared to the two recombinant pseudotype baculoviruses. Conclusions These results indicate that pseudotype baculovirus-mediated gene delivery could be a alternative strategy to develop a new generation of vaccines against FMDV infection. |
| Databáze: | OpenAIRE |
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