Ceramide-1-phosphate, a new mediator of development and survival in retina photoreceptors
| Autor: | Luis E. Politi, Nora P. Rotstein, Gisela Edit Miranda, Carolina E. Abrahan, Daniela L. Agnolazza |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2011 |
| Předmět: |
Opsin
Ceramide genetic structures Cell Survival Biología Blotting Western Peripherins Apoptosis Nerve Tissue Proteins Biología Celular Ceramides Retina chemistry.chemical_compound Intermediate Filament Proteins Annexin In Situ Nick-End Labeling medicine Animals DAPI Propidium iodide Rats Wistar Fluorescent Antibody Technique Indirect Ceramide-1-phosphate Cells Cultured Cell Proliferation Membrane Potential Mitochondrial Sphingolipids Membrane Glycoproteins Opsins Sphingosine Stem Cells Cell Differentiation Peripherin eye diseases Rats Cell biology medicine.anatomical_structure Microscopy Fluorescence chemistry sense organs Photoreceptor Cells Vertebrate |
| Zdroj: | SEDICI (UNLP) Universidad Nacional de La Plata instacron:UNLP |
| Popis: | Purpose. Simple sphingolipids control crucial cellular processes in several cell types. Previous work demonstrated that sphingolipids, such as ceramide, sphingosine, and sphingosine-1-phosphate, are key mediators in the regulation of survival, differentiation, and proliferation of retina photoreceptors. Ceramide-1-phosphate (C1P) regulates growth and survival in several cell types; however, little is known concerning its functions in the retina. Whether C1P also participates in controlling photoreceptor development was also explored. Methods. Rat retina neuronal cultures were supplemented with 1 to 10 μM C1P. Proliferation was determined by evaluating 5-bromo-2-deoxyuridine (BrdU) uptake and the number of mitotic figures and differentiation by evaluating opsin and peripherin expression by immunocytochemistry and Western blot. Apoptosis was inhibited with the pan caspase inhibitor ZVADFMK and evaluated by TUNEL assay, propidium iodide/annexin V, and DAPI labeling. Preservation of mitochondrial membrane potential was evaluated. Results. C1P enhanced BrdU uptake and increased mitosis in retinal progenitors. C1P addition advanced photoreceptor differentiation, enhancing opsin and peripherin expression and stimulating development of the apical processes in which these proteins were concentrated. In the absence of these trophic factors, photoreceptors degenerated after 4 days in vitro, and at day 6, almost 50% of photoreceptors were apoptotic. C1P decreased photoreceptor apoptosis, reducing this percentage by half. Inhibiting caspase activity reduced photoreceptor apoptosis in the controls, but did not increase opsin expression, implying that C1P has separate effects on differentiation and survival. Conclusions. These results suggest for the first time that C1P is a novel mediator that has multiple functions in photoreceptors, initially regulating their proliferation and then promoting their survival and differentiation. Instituto Multidisciplinario de Biología Celular |
| Databáze: | OpenAIRE |
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