Structure-cytotoxicity relationship of methacrylate-based resin monomers as evaluated by an anti-oxidant responsive element-luciferase reporter assay
| Autor: | Hiroshi Nakamura, Tohru Obata, Takao Kanamori, Miki Egashira, Motohiro Tanaka, Tatsushi Kawai, Ai Orimoto, Takahiro Suzuki |
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| Rok vydání: | 2016 |
| Předmět: |
Materials science
02 engineering and technology Methacrylate law.invention 03 medical and health sciences chemistry.chemical_compound 0302 clinical medicine Magazine law Genes Reporter Polymer chemistry Humans Luciferase Cytotoxicity Luciferases General Dentistry chemistry.chemical_classification 030206 dentistry Fibroblasts 021001 nanoscience & nanotechnology Oxidants Resins Synthetic Monomer Hydrocarbon chemistry Electrophile Ceramics and Composites Methacrylates 0210 nano-technology Intracellular |
| Zdroj: | Dental materials journal. 35(6) |
| ISSN: | 1881-1361 |
| Popis: | To investigate the chemical structure-cytotoxicity relationship of methacrylate-based resin monomers, we studied their effects on anti-oxidant responsive element (ARE)-mediated transcription. HepG2 cells stably expressing an ARE-regulated luciferase reporter gene were cultured for 6 h with various concentrations of several resin monomers and subjected to a luciferase assay. The doseresponse curves observed for hydrophobic monomers with different hydrocarbon chains (MMA, EMA, PMA and BMA) began to rise at concentrations between 0.5 and 1 mM; the curves rose as the monomer concentrations increased up to 5 (BMA), 10 (PMA), or 30 mM (MMA and EMA). In contrast, hydrophilic monomers having a hydroxyl group (HEMA and HPMA) showed bell-shaped curves, and stimulated the reporter expression more strongly than the hydrophobic monomers in a low concentration range (0.5-5 mM). The results suggest that introduction of a hydroxyl group in a methacrylate-based resin monomer increases its intracellular electrophilic reactivity and cytotoxicity. |
| Databáze: | OpenAIRE |
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