Topically Applied Diacylglycerols Increase Pigmentation in Guinea Pig Skin
| Autor: | Barbara A. Gilchrest, Elizabeth P. Messana, Anne E. Allan, Michael Archambault |
|---|---|
| Rok vydání: | 1995 |
| Předmět: |
medicine.medical_specialty
Ultraviolet Rays Administration Topical Guinea Pigs Skin Pigmentation Dermatology Melanocyte Biology Biochemistry Diglycerides Melanin Guinea pig In vivo Internal medicine medicine Animals Humans Molecular Biology Protein kinase C Skin Diacylglycerol kinase Melanins Dose-Response Relationship Drug Cell Biology In vitro Dose–response relationship Endocrinology medicine.anatomical_structure Melanocytes |
| Zdroj: | Journal of Investigative Dermatology. 105:687-692 |
| ISSN: | 0022-202X |
| DOI: | 10.1111/1523-1747.ep12324466 |
| Popis: | Exposure of human and murine melanocytes in vitro to the diacylglycerol (DAG) 1-oleoyl-2-acetyl-sn-glycerol (OAG) markedly increases melanin production within 24 h. To determine whether OAG can increase melanin production in vivo , increasing concentrations of OAG (10–60 mg/ml) in propylene glycol were applied daily for 5 d to shaved guinea pigs. Dose-dependent increased pigmentation was visible first on days 17–22 and persisted for 10–14 weeks. Peak epidermal melanin content in OAG-treated sites was more than twice that of untreated or vehicle-treated sites, as assessed by computerized image analysis of Fontana-Masson stained biopsy cross sections. In another experiment to assess the mechanism of DAG-mediated pigmentation, guinea pigs received twice daily separate applications of OAG, dipalmitoylglycerol (diC 16 ), dioctanoylglycerol (diC 8 ), each 50 mg/ml, 20 μl/application, and propylene glycol vehicle alone for 5 d. Increased pigmentation was visible after 10 d in the OAG and diC 8 sites but not in diC 16 or vehicle sites. These results correlate with the reported ability of these compounds to activate protein kinase C in vitro . In a final experiment, guinea pigs received OAG 25 mg/ml three times daily to one test site, and once daily ultraviolet B (70 mJ/cm 2 , equivalent to 0.6 minimal erythemal dose) radiation to another for 10 d. The OAG and ultraviolet B test sites developed comparable pigmentation by both clinical and histologic criteria. Our data demonstrate that topically applied DAGs can produce a long-lasting increase in epidermal pigmentation, presumably through protein kinase C activation, which clinically and histologically closely resembles ultraviolet-induced tanning. |
| Databáze: | OpenAIRE |
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