Camera-based three-dimensional real-time particle tracking at kHz rates and Ångström accuracy
| Autor: | Peter Daldrop, Daniel Klaue, Ralf Seidel, Hergen Brutzer, Oliver Otto, Alexander Huhle, Sihwa Joo, Ulrich F. Keyser |
|---|---|
| Přispěvatelé: | Keyser, Ulrich [0000-0003-3188-5414], Apollo - University of Cambridge Repository |
| Jazyk: | angličtina |
| Rok vydání: | 2015 |
| Předmět: |
Physics
Magnetic tweezers Data processing FOS: Nanotechnology Multidisciplinary business.industry 0299 Other Physical Sciences Resolution (electron density) General Physics and Astronomy Nanotechnology Bioengineering General Chemistry Tracking (particle physics) General Biochemistry Genetics and Molecular Biology Article Photodiode law.invention Brain Disorders Optics Optical tweezers law Particle Angstrom business |
| Zdroj: | Nature communications |
| ISSN: | 2041-1723 |
| Popis: | Optical and magnetic tweezers are widely employed to probe the mechanics and activity of individual biomolecular complexes. They rely on micrometre-sized particles to detect molecular conformational changes from the particle position. Real-time particle tracking with Ångström accuracy has so far been only achieved using laser detection through photodiodes. Here we demonstrate that camera-based imaging can provide a similar performance for all three dimensions. Particle imaging at kHz rates is combined, with real-time data processing being accelerated by a graphics-processing unit. For particles that are fixed in the sample cell we can detect 3-Å-sized steps that are introduced by cell translations at rates of 10 Hz, while for DNA-tethered particles 5 Å steps at 1 Hz can be resolved. Moreover, 20 particles can be tracked in parallel with comparable accuracy. Our approach provides a simple and robust way for high-resolution tweezer experiments using multiple particles at a time. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|