Role of cytochrome P-450 in schisandrin B-induced antioxidant and heat shock responses in mouse liver
| Autor: | Duncan H. Mak, Kam Ming Ko, Michel Kwong Tat Poon, Po Yee Chiu |
|---|---|
| Rok vydání: | 2005 |
| Předmět: |
Antioxidant
Cytochrome medicine.medical_treatment Administration Oral Mitochondria Liver medicine.disease_cause Antioxidants Lignans General Biochemistry Genetics and Molecular Biology Cyclooctanes Mice chemistry.chemical_compound Cytochrome P-450 Enzyme System Heat shock protein medicine Animals Cytochrome P-450 Enzyme Inhibitors Polycyclic Compounds Enzyme Inhibitors General Pharmacology Toxicology and Pharmaceutics Heat shock Mice Inbred BALB C Dose-Response Relationship Drug biology General Medicine Glutathione Triazoles Molecular biology Oxidative Stress Liver chemistry Microsomes Liver biology.protein Microsome Female Schisandraceae Heat-Shock Response NADP Oxidative stress |
| Zdroj: | Life Sciences. 77:2887-2895 |
| ISSN: | 0024-3205 |
| DOI: | 10.1016/j.lfs.2005.03.026 |
| Popis: | In order to explore the role of cytochrome P-450 (P-450) in schisandrin B (Sch B)-induced antioxidant and heat shock responses, the effect of 1-aminobenzotriazole (ABT, a broad spectrum inhibitor of P-450) on hepatic mitochondrial glutathione antioxidant status (mtGAS) and heat shock protein (Hsp)25/70 expression was examined in Sch B-treated mice. The non-specific and partial inhibition of cytochrome P-450 (P-450) by ABT pretreatment significantly caused a protraction in the time-course of Sch B-induced enhancement in hepatic mitGAS and Hsp25/70 expression in mice. Using mouse liver microsomes as a source of P-450, Sch B, but not dimethyl diphenyl bicarboxylate (a non-hepatoprotective analog of Sch B), was found to serve as a co-substrate for the P-450-catalyzed NADPH oxidation reaction, with a concomitant production of oxidant species. Taken together, the results suggest that oxidant species generated from P-450-catalyzed reaction with Sch B may trigger the antioxidant and heat shock responses in mouse liver. |
| Databáze: | OpenAIRE |
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