GC/MS and LC/MS Based Serum Metabolomic Analysis of Dairy Cows With Ovarian Inactivity
| Autor: | Shixin Fu, Jiang Zhang, Ling Wu, Cheng Xia, Yuxi Song, Yunlong Bai, Chuang Xu |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2021 |
| Předmět: |
Estrous cycle
medicine.medical_specialty General Veterinary Veterinary medicine Retinol Ovary Lipid metabolism Metabolism Carbohydrate metabolism Steroid biosynthesis chemistry.chemical_compound medicine.anatomical_structure Endocrinology chemistry UHPLC-QTOF-MS Internal medicine SF600-1100 GC-TOF-MS Follicular phase medicine Veterinary Science dairy cows inactive ovaries metabolism Original Research |
| Zdroj: | Frontiers in Veterinary Science Frontiers in Veterinary Science, Vol 8 (2021) |
| ISSN: | 2297-1769 |
| DOI: | 10.3389/fvets.2021.678388 |
| Popis: | Metabolic disorders may lead to the inactive ovaries of dairy cows during early lactation. However, the detailed metabolic profile of dairy cows with inactive ovaries around 55 days postpartum has not been clearly elucidated. The objective of this study was to investigate the metabolic difference in cows with inactive ovaries and estrus from the perspective of serum metabolites. According to clinical manifestations, B-ultrasound scan, rectal examination, 15 cows were assigned to the estrus group (E; follicular diameter 15–20 mm) and 15 to the inactive ovary group (IO; follicular diameter p = 0.0012), 9-cis-retinal (p = 0.0030), oxamic acid (p = 0.0321), etc.) while 10 metabolites were significantly lower (monostearin (p = 0.0001), 3-hydroxypropionic acid (p = 0.0005), D-talose (p = 0.0018), etc.). Pathway analysis indicated that the serum differential metabolites of multiparous cows in estrus obtained by the two metabolomics techniques were mainly involved in β-alanine metabolism and steroid biosynthesis metabolism, while other involved metabolic pathways were related to metabolism of glyoxylate; dicarboxylate metabolism; fructose, mannose, glutathione, glycerolipid, glycine, serine, threonine, propanoate, retinol, and pyrimidine metabolism. This indicates that the abnormalities in glucose metabolism, lipid metabolism, amino acid metabolism, and glutathione metabolism of postpartum dairy cows obstructed follicular development. |
| Databáze: | OpenAIRE |
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