Control of Swe1p degradation by the morphogenesis checkpoint
| Autor: | Rey A. L. Sia, Daniel J. Lew, Elaine S.G. Bardes |
|---|---|
| Rok vydání: | 1998 |
| Předmět: |
Saccharomyces cerevisiae Proteins
Cell cycle checkpoint Saccharomyces cerevisiae Cell Cycle Proteins Biology General Biochemistry Genetics and Molecular Biology Proto-Oncogene Proteins c-myc Morphogenesis CHEK1 Phosphorylation Molecular Biology Mitosis DNA Primers Base Sequence General Immunology and Microbiology Hydrolysis General Neuroscience Cell Cycle Protein-Tyrosine Kinases G2-M DNA damage checkpoint Cell cycle biology.organism_classification Actin cytoskeleton Cell biology Wee1 biology.protein CDC28 Protein Kinase S cerevisiae Research Article |
| Zdroj: | The EMBO Journal. 17:6678-6688 |
| ISSN: | 1460-2075 |
| DOI: | 10.1093/emboj/17.22.6678 |
| Popis: | In the budding yeast Saccharomyces cerevisiae, a cell cycle checkpoint coordinates mitosis with bud formation. Perturbations that transiently depolarize the actin cytoskeleton cause delays in bud formation, and a 'morphogenesis checkpoint' detects the actin perturbation and imposes a G2 delay through inhibition of the cyclin-dependent kinase, Cdc28p. The tyrosine kinase Swe1p, homologous to wee1 in fission yeast, is required for the checkpoint-mediated G2 delay. In this report, we show that Swe1p stability is regulated both during the normal cell cycle and in response to the checkpoint. Swe1p is stable during G1 and accumulates to a peak at the end of S phase or in early G2, when it becomes unstable and is degraded rapidly. Destabilization of Swe1p in G2 and M phase depends on the activity of Cdc28p in complexes with B-type cyclins. Several different perturbations of actin organization all prevent Swe1p degradation, leading to the persistence or further accumulation of Swe1p, and cell cycle delay in G2. |
| Databáze: | OpenAIRE |
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