Biochemical characterization and antifungal activity of an endo-1,3-β-glucanase of Paenibacillus sp. isolated from garden soil
| Autor: | T.-Y. Hong, Menghsiao Meng |
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| Rok vydání: | 2003 |
| Předmět: |
DNA
Bacterial Antifungal Agents Microbial Sensitivity Tests Polysaccharide DNA Ribosomal Applied Microbiology and Biotechnology Substrate Specificity Microbiology Paenibacillus Amino Acid Sequence Pythium aphanidermatum Bacillaceae Soil Microbiology Mycelium chemistry.chemical_classification Base Sequence Sequence Homology Amino Acid biology Glucan Endo-1 3-beta-D-Glucosidase food and beverages General Medicine Streptomyces sioyaensis Glucanase biology.organism_classification Xylan Molecular Weight Kinetics Enzyme Biochemistry chemistry Biotechnology |
| Zdroj: | Applied Microbiology and Biotechnology. 61:472-478 |
| ISSN: | 1432-0614 0175-7598 |
| DOI: | 10.1007/s00253-003-1249-z |
| Popis: | A 44-kDa 1,3-beta-glucanase was purified from the culture medium of a Paenibacillus strain with a 28-fold increase in specific activity with 31% recovery. The purified enzyme preferentially catalyzes the hydrolysis of glucans with 1,3-beta-linkage and has an endolytic mode of action. The enzyme also showed binding activity to various insoluble polysaccharides including unhydrolyzable substrates such as xylan and cellulose. The antifungal activity of this Paenibacillus enzyme and a previously purified 1,3-beta-glucanase from Streptomyces sioyaensis were examined in this study. Both enzymes had the ability to damage the cell-wall structures of the growing mycelia of phytopathogenic fungi Pythium aphanidermatum and Rhizoctonic solani AG-4. Nonetheless, the Paenibacillus enzyme had a much stronger effect on inhibiting the growth of fungi tested. |
| Databáze: | OpenAIRE |
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