Lung endothelial barrier protection by resveratrol involves inhibition of HMGB1 release and HMGB1-induced mitochondrial oxidative damage via an Nrf2-dependent mechanism
| Autor: | Yu-Jian Liu, Xiaoyan Zhu, Yan-Fei Mao, Lai Jiang, Zhou Lv, Ying-Wei Wang, Wen-Wen Dong |
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| Rok vydání: | 2015 |
| Předmět: |
Male
NF-E2-Related Factor 2 Ventilator-Induced Lung Injury Blotting Western SOD2 Fluorescent Antibody Technique chemical and pharmacologic phenomena Enzyme-Linked Immunosorbent Assay Resveratrol Pharmacology Lung injury HMGB1 Real-Time Polymerase Chain Reaction Transfection Biochemistry Antioxidants chemistry.chemical_compound Mice Downregulation and upregulation In vivo Physiology (medical) Stilbenes Animals DAPI HMGB1 Protein RNA Small Interfering Lung Gene knockdown Mice Inbred ICR biology Endothelial Cells respiratory system Flow Cytometry Mitochondria Disease Models Animal Oxidative Stress chemistry Gene Knockdown Techniques biology.protein Stress Mechanical |
| Zdroj: | Free radical biologymedicine. 88 |
| ISSN: | 1873-4596 |
| Popis: | High-mobility group box 1 (HMGB1) contributes to lung vascular hyperpermeability during ventilator-induced lung injury. We aimed to determine whether the natural antioxidant resveratrol protected against HMGB1-induced endothelial hyperpermeability both in vitro and in vivo. We found that HMGB1 decreased vascular endothelial (VE)-cadherin expression and increased endothelial permeability, leading to mitochondrial oxidative damage in primary cultured mouse lung vascular endothelial cells (MLVECs). Both the mitochondrial superoxide dismutase 2 mimetic MnTBAP and resveratrol blocked HMGB1-induced mitochondrial oxidative damage, VE-cadherin downregulation, and endothelial hyperpermeability. In in vivo studies, anesthetized male ICR mice were ventilated for 4 h using low tidal volume (6 ml/kg) or high tidal volume (HVT; 30 ml/kg) ventilation. The mice were injected intraperitoneally with resveratrol immediately before the onset of ventilation. We found that resveratrol attenuated HVT-associated lung vascular hyperpermeability and HMGB1 production. HVT caused a significant increase in nuclear factor-erythroid 2-related factor 2 (Nrf2) nuclear translocation and Nrf2 target gene expression in lung tissues, which was further enhanced by resveratrol treatment. HMGB1 had no effect on Nrf2 activation, whereas resveratrol treatment activated the Nrf2 signaling pathway in HMGB1-treated MLVECs. Moreover, Nrf2 knockdown reversed the inhibitory effects of resveratrol on HMGB1-induced mitochondrial oxidative damage and endothelial hyperpermeability. The inhibitory effect of resveratrol on cyclic stretch-induced HMGB1 mRNA expression in primary cultured MLVECs was also abolished by Nrf2 knockdown. In summary, this study demonstrates that resveratrol protects against lung endothelial barrier dysfunction initiated by HVT. Lung endothelial barrier protection by resveratrol involves inhibition of mechanical stretch-induced HMGB1 release and HMGB1-induced mitochondrial oxidative damage. These protective effects of resveratrol might be mediated through an Nrf2-dependent mechanism. |
| Databáze: | OpenAIRE |
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