Evaluation of the Osteogenic Potential of Different Scaffolds Embedded with Human Stem Cells Originated from Schneiderian Membrane: An In Vitro Study
| Autor: | Bassam Badran, Rawan Makki, Fatima Al-Nemer, Mohammad Fayyad-Kazan, Hussein Fayyad-Kazan, Rita Bou Assaf, Antoine Berberi |
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| Rok vydání: | 2019 |
| Předmět: |
food.ingredient
Article Subject Cell Survival Cell lcsh:Medicine 02 engineering and technology Schneiderian Membrane Gelatin Bone and Bones General Biochemistry Genetics and Molecular Biology 03 medical and health sciences 0302 clinical medicine food Osteogenesis medicine Humans MTT assay Viability assay Cells Cultured Osteoblasts Tissue Engineering Tissue Scaffolds General Immunology and Microbiology Chemistry lcsh:R Mesenchymal stem cell Cell Differentiation Mesenchymal Stem Cells 030206 dentistry General Medicine Alkaline Phosphatase 021001 nanoscience & nanotechnology Cell biology Nasal Mucosa medicine.anatomical_structure Alkaline phosphatase Collagen Stem cell 0210 nano-technology Research Article |
| Zdroj: | BioMed Research International BioMed Research International, Vol 2019 (2019) |
| ISSN: | 2314-6141 2314-6133 |
| Popis: | Background. Novel treatments for bone defects, particularly in patients with poor regenerative capacity, are based on bone tissue engineering strategies which include mesenchymal stem cells (MSCs), bioactive factors, and convenient scaffold supports. Objective. In this study, we aimed at comparing the potential for different scaffolds to induce osteogenic differentiation of human maxillary Schneiderian sinus membrane- (hMSSM-) derived cells. Methods. hMSSM-derived cells were seeded on gelatin, collagen, or Hydroxyapatite β-Tricalcium phosphate-Fibrin (Haβ-TCP-Fibrin) scaffolds. Cell viability was determined using an MTT assay. Alizarin red staining method, Alkaline phosphatase (ALP) activity assay, and quantitative real-time PCR analysis were performed to assess hMSSM-derived cells osteogenic differentiation. Results. Cell viability, calcium deposition, ALP activity, and osteoblastic markers transcription levels were most striking in gelatin scaffold-embedded hMSSM-derived cells. Conclusion. Our findings suggest a promising potential for gelatin-hMSSM-derived cell construct for treating bone defects. |
| Databáze: | OpenAIRE |
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