Comparison of two fluorescent probes in preclinical non-invasive imaging and image-guided debridement surgery of Staphylococcal biofilm implant infections
| Autor: | Vishal Hegde, Lloyd S. Miller, Kevin P. Francis, Julie Czupryna, John Hoang, Mafalda Bispo, Zachary D. C. Burke, Marina López-Álvarez, Jan Maarten van Dijl, Francisco Romero Pastrana, Christopher D. Hamad, William L. Sheppard, Marleen van Oosten, Ryan Smith, Nicholas M. Bernthal, Marina M. Sprague, Stephen D. Zoller, Gideon Blumstein, Howard Y. Park |
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| Přispěvatelé: | Translational Immunology Groningen (TRIGR), Microbes in Health and Disease (MHD), University of Groningen |
| Rok vydání: | 2021 |
| Předmět: |
0301 basic medicine
medicine.medical_treatment medicine.disease_cause Mice Biofilms/drug effects Computer-Assisted Medicine Tomography Multidisciplinary Optical Imaging Vancomycin/pharmacology Prostheses and Implants Fluorescence Anti-Bacterial Agents X-Ray Computed Infectious Diseases Surgery Computer-Assisted Spinal Cord Staphylococcus aureus Infectious diseases Infection Noninvasive imaging medicine.medical_specialty Prosthesis-Related Infections Science 030106 microbiology Bioengineering Fluorescent imaging Article Anti-Bacterial Agents/pharmacology 03 medical and health sciences Spinal Cord/diagnostic imaging Vancomycin Animals Fluorescent Dyes/chemistry Fluorescent Dyes Debridement business.industry Animal Biofilm Implant Infection Prosthesis-Related Infections/drug therapy Optical Imaging/methods Surgery Disease Models Animal 030104 developmental biology Emerging Infectious Diseases Staphylococcus aureus/isolation & purification Biofilms Disease Models Implant business Tomography X-Ray Computed |
| Zdroj: | Scientific reports, vol 11, iss 1 Scientific Reports, 11(1):1622. Nature Publishing Group Scientific Reports, 11(1). Nature Publishing Group Scientific Reports Scientific Reports, Vol 11, Iss 1, Pp 1-13 (2021) |
| ISSN: | 2045-2322 |
| Popis: | Implant-associated infections are challenging to diagnose and treat. Fluorescent probes have been heralded as a technologic advancement that can improve our ability to non-invasively identify infecting organisms, as well as guide the inexact procedure of surgical debridement. This study’s purpose was to compare two fluorescent probes for their ability to localize Staphylococcus aureus biofilm infections on spinal implants utilizing noninvasive optical imaging, then assessing the broader applicability of the more successful probe in other infection animal models. This was followed by real-time, fluorescence image-guided surgery to facilitate debridement of infected tissue. The two probe candidates, a labelled antibiotic that targets peptidoglycan (Vanco-800CW), and the other, a labelled antibody targeting the immunodominant Staphylococcal antigen A (1D9-680), were injected into mice with spine implant infections. Mice were then imaged noninvasively with near infrared fluorescent imaging at wavelengths corresponding to the two probe candidates. Both probes localized to the infection, with the 1D9-680 probe showing greater fidelity over time. The 1D9-680 probe was then tested in mouse models of shoulder implant and allograft infection, demonstrating its broader applicability. Finally, an image-guided surgery system which superimposes fluorescent signals over analog, real-time, tissue images was employed to facilitate debridement of fluorescent-labelled bacteria. |
| Databáze: | OpenAIRE |
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