Transcriptomic Prediction of Pig Liver-Enriched Gene 1 Functions in a Liver Cell Line
| Autor: | Yanna Dang, Jin-Yang Wang, Zhe Zhang, Zizengchen Wang, Huanan Wang, Sakthidasan Jayaprakash, Jin He |
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| Jazyk: | angličtina |
| Rok vydání: | 2020 |
| Předmět: |
0301 basic medicine
pig Carcinoma Hepatocellular lcsh:QH426-470 Transcriptome 03 medical and health sciences LEG1 biology.animal Genetics Biomarkers Tumor Tumor Cells Cultured Humans Gene Zebrafish Genetics (clinical) 030102 biochemistry & molecular biology biology Liver cell Endoplasmic reticulum Communication Liver Neoplasms Computational Biology Proteins biology.organism_classification Cell biology Gene Expression Regulation Neoplastic lcsh:Genetics 030104 developmental biology functional prediction Subfunctionalization Domain of unknown function RNA-seq Platypus |
| Zdroj: | Genes Genes, Vol 11, Iss 412, p 412 (2020) |
| ISSN: | 2073-4425 |
| Popis: | The newly identified liver-enriched gene 1 (LEG1) encodes a protein with a characteristic domain of unknown function 781 (DUF781/LEG1), constituting a protein family with only one member in mammals. A functional study in zebrafish suggested that LEG1 genes are involved in liver development, while the platypus LEG1 homolog, Monotreme Lactation Protein (MLP), which is enriched in the mammary gland and milk, acts as an antibacterial substance. However, no functional studies on eutherian LEG1s have been published to date. Thus, we here report the first functional prediction study at the cellular level. As previously reported, eutherian LEG1s can be classified into three paralogous groups. Pigs have all three LEG1 genes (pLEG1s), while humans and mice have retained only LEG1a. Hence, pLEG1s might represent an ideal model for studying LEG1 gene functions. RNA-seq was performed by the overexpression of pLEG1s and platypus MLP in HepG2 cells. Enrichment analysis showed that pLEG1a and pLEG1b might exhibit little function in liver cells; however, pLEG1c is probably involved in the endoplasmic reticulum (ER) stress response and protein folding. Additionally, gene set enrichment analysis revealed that platypus MLP shows antibacterial activity, confirming the functional study in platypus. Therefore, our study showed from the transcriptomic perspective that mammalian LEG1s have different functions in liver cells due to the subfunctionalization of paralogous genes. |
| Databáze: | OpenAIRE |
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