Diversitat i supervivència de bacteris aeris al litoral del mar Mediterrani
| Autor: | Sastre Barrera, Marc |
|---|---|
| Přispěvatelé: | Sala, M. Montserrat, Urmeneta, Jordi |
| Jazyk: | Catalan; Valencian |
| Rok vydání: | 2019 |
| Předmět: | |
| Zdroj: | Digital.CSIC. Repositorio Institucional del CSIC instname |
| Popis: | Trabajo final presentado por Marc Sastre Barrera para el grado de Biotecnología en la Universitat de Barcelona (UB), realizado bajo la dirección de la Dra. Maria Montserrat Solé Rovira del Institut de Ciències del Mar (ICM-CSIC) y del Dr.Jordi Urmeneta de la Universitat de Barcelona (UB).-- 36 pages, 18 figures, annexes The diversity and survival of airborne bacterial communities in NW Mediterranean coastal water was studied in a mesocosm experiment. Treatments consisted of mesocosms filled with 1) seawater, 2) seawater filtered through 0.8 um to avoid the presence of bacterial predators, and 3) artificial seawater. Atmospheric deposition was collected (CSIC) for 11 days and added to half of the containers (D) while the other half were left unamended. During the experiment we followed the composition of the bacterial community by catalyzed reporter in situ hybridization (CARD-FISH), a microscopical technique that allows the quantification of bacterial groups. The four probes used were selected for targeting groups relevant in marine or airborne bacteria: Eubacteria, Firmicutes, SAR11 and Betaproteobacteria. Our results show that a significant amount of bacteria was added with the deposition (3,67·105 bacteria·mL-1), which had a different composition than that of the seawater community, which had predominantly marine bacteria SAR11 and a lower percentage of Firmicutes and Betaproteobacteria. The viability along time of this groups was very low. In spite of the increase in bacterial community in predator-free seawater after deposition, none of the groups followed increased in concentration. This is very likely due to an increase of Gammaproteobacteria, which was not targeted with our set of probes, an opportunistic group, either of atmospheric or marine origin, that might have quickly responded to the addition of nutrients with the deposition. The most abundant marine bacteria SAR11 increased in the SW treatments but not in the predator-free treatment, probably due to its small size that can easily escape predation and can become abundant in experiments in the absence of other larger opportunistic bacteria such as Gammaproteobacteria which might be dominant in the predator-free treatments. Our experiment has shown a very limited survival of airborne microorganisms in seawater |
| Databáze: | OpenAIRE |
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