Functional Analysis of a Brassica oleracea SLR1 Gene Promoter
| Autor: | G. Cadwallader, F. C. H. Franklin, R. M. Hackett |
|---|---|
| Rok vydání: | 1996 |
| Předmět: |
DNA
Plant Physiology Recombinant Fusion Proteins Transgene Molecular Sequence Data Brassica Plant Science Biology Transformation Genetic Gene expression Genetics Promoter Regions Genetic Gene Glucuronidase Plant Proteins Reporter gene Base Sequence food and beverages Promoter biology.organism_classification Molecular biology Transformation (genetics) Regulatory sequence Brassica oleracea Research Article |
| Zdroj: | Plant Physiology. 112:1601-1607 |
| ISSN: | 1532-2548 0032-0889 |
| DOI: | 10.1104/pp.112.4.1601 |
| Popis: | The Brassica oleracea S-locus-related gene 1 (SLR1) is expressed in the papillar cells of Brassica stigmas from a few days before anthesis. We have previously shown that a 1500-bp fragment of the SLR1 gene promoter is sufficient to direct high-level, temporally regulated expression of the β-glucuronidase reporter gene in the pistils of transgenic tobacco. We have carried out a deletion analysis of the SLR1 promoter and found that elements required for pistil expression are located between -258 and -327 bp (relative to the translation start site). Furthermore, specific binding of pistil nuclear factors to sequences within this region was demonstrated by gel retardation analysis. Sequences between -1350 and -1500 were found to be required for high-level expression. |
| Databáze: | OpenAIRE |
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