Sp1-like proteins function in the transcription of human ferredoxin genes

Autor: Jing Ruey Yeh, Bon Chu Chung, Li-Chung Hsu
Rok vydání: 2000
Předmět:
TBX1
Transcription
Genetic
Sp1 Transcription Factor
Recombinant Fusion Proteins
Endocrinology
Diabetes and Metabolism
Clinical Biochemistry
Response element
Regulatory Sequences
Nucleic Acid
Biology
Transfection
Second Messenger Systems
Mice
Transcription (biology)
Cyclic AMP
Tumor Cells
Cultured
Animals
Humans
Pharmacology (medical)
Cholesterol Side-Chain Cleavage Enzyme
Phosphorylation
Enhancer
Protein kinase A
Molecular Biology
Gene
Ferredoxin
Colforsin
Genetic Complementation Test
Biochemistry (medical)
Cytochrome P450
Cell Biology
General Medicine
Cyclic AMP-Dependent Protein Kinases
Molecular biology
Adrenal Cortex Neoplasms
Neoplasm Proteins
Biochemistry
Multigene Family
Adrenal Cortex
biology.protein
Ferredoxins
Steroid 21-Hydroxylase
Protein Processing
Post-Translational
Protein Binding
Transcription Factors
Zdroj: Journal of Biomedical Science. 7:144-151
ISSN: 1423-0127
1021-7770
DOI: 10.1007/bf02256621
Popis: We characterized a regulatory element located in the -76 to -62 region of the human ferredoxin gene. This region bound to Sp1-like proteins with low affinity, as shown using electrophoretic mobility shift, competition, antibody binding, and Southwestern experiments. The similarity of the regulatory element to Sp1 extends beyond its DNA-binding domain, as cloned Sp1 functioned equally well when fused to a peptide that bound to an irrelevant site. The function of these Sp1-binding sites is mediated through the cAMP-dependent protein kinase (PKA) signaling pathway, because reporter genes downstream of the Sp1-binding sites were not activated in a PKA-deficient cell line. Transfection of the catalytic subunit of PKA restored activated transcription. Similar Sp1-binding sites identified in the CYP11A1 and CYP21 genes also controlled cAMP-dependent transcription of the reporter gene. Our finding of the function of Sp1-like proteins in steroidogenic gene transcription adds one more role Sp1 plays in controlling physiological events.
Databáze: OpenAIRE
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