UTILIZAÇÃO DE NITRATO DE AMÔNIO E DE URÉIA COMO FONTES DE NITROGÊNIO NA MICROPROPAGAÇÃO DE AMOREIRA-PRETA
| Autor: | Moacir Pasqual, Leila Aparecida Salles Pio, Fabíola Villa, Juliana Costa de Rezende, Chrystiane Borges Fráguas |
|---|---|
| Přispěvatelé: | Fundação de Amparo à Pesquisa de Minas Gerais (FAPEMIG), Universidade Federal de Lavras (UFLA), Universidade Estadual Paulista (Unesp), Empresa de Pesquisa Agropecuária de Minas Gerais (EPAMIG) |
| Rok vydání: | 2009 |
| Předmět: |
food.ingredient
biology Nitrogen Inoculation Culture media MS Ammonium nitrate Soil Science chemistry.chemical_element Plant Science biology.organism_classification chemistry.chemical_compound Horticulture Rubus sp food chemistry Urea Agar Rubus Agronomy and Crop Science Completely randomized design Explant culture |
| Zdroj: | Scopus Repositório Institucional da UNESP Universidade Estadual Paulista (UNESP) instacron:UNESP |
| ISSN: | 1983-2443 1519-1125 |
| DOI: | 10.5380/rsa.v10i5.15192 |
| Popis: | Submitted by Vitor Silverio Rodrigues (vitorsrodrigues@reitoria.unesp.br) on 2014-05-27T11:23:58Z No. of bitstreams: 0Bitstream added on 2014-05-27T14:46:31Z : No. of bitstreams: 1 2-s2.0-84871627919.pdf: 281107 bytes, checksum: b0c8e203e5d22bf25532bae8392fddbf (MD5) Made available in DSpace on 2014-05-27T11:23:58Z (GMT). No. of bitstreams: 0 Previous issue date: 2009-09-01 Fundação de Amparo à Pesquisa do Estado de Minas Gerais (FAPEMIG) The high cost allied to the difficulty in the acquisition of the ammonium nitrate has been taking the accomplishment of works looking for an alternative for the substitution of this source of nitrogen. It was aimed at to study the technical viability of the substitution of the ammonium nitrate for urea, as source of nitrogen in the culture media for the blackberry in vitro cv. Tupy (Rubus sp). Nodal segments were used, already established in vitro, were inoculated in culture media MS and 50% MS, added of 1.0 mg. dm-3 of BAP, solidified with 6 g.dm-3 of agar, pH was adjusted for 5,8 and sterilized to 121 °C and 0,1 MPa for 20 min. The treatments consisted of the substitution of 0; 20; 40; 60; 80 and 100% of NH4NO3 for urea, and the swinging of nitrogen supplied by the culture media MS it was not altered. The explants were maintained by 60 days in growth room with temperature of 27±1 °C, photoperiod of 16 h and luminous intensity of 32 mmol m'2 s'1. The experiment was arranged in a completely randomized design, in factorial (6 x 2) using four repetitions with 16 plants each one. From the obtained results it can be concluded that the urea doesn't substitute NH 4NO3 in the culture media MS as nitrogen source in the culture vitro of blackberry. Empresa de Pesquisa Agropecuária de Minas (EPAMIG) Fundação de Amparo à Pesquisa de Minas Gerais (FAPEMIG), Av. Washington Viglione, s/n, Maria da Fé, Minas Gerais, CEP: 37517-000 Departamento de Agricultura (DAG) Universidade Federal de Lavras (UFLA), Lavras, Minas Gerais, CEP 37200-000 Departamento de Biologia Universidade Federal de Lavras (UFLA), Lavras, Minas Gerais Faculdade de Ciências Agrárias e Veterinárias (FCAV) Universidade Estadual Paulista (UNESP), Botucatu, São Paulo Pesquisadora da Empresa de Pesquisa Agropecuária de Minas Gerais (EPAMIG), Lavras, Minas Gerais Faculdade de Ciências Agrárias e Veterinárias (FCAV) Universidade Estadual Paulista (UNESP), Botucatu, São Paulo |
| Databáze: | OpenAIRE |
| Externí odkaz: |
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