Hapten-Specific T Cell Response to Azobenzenearsonate-N-Acetyl-L-Tyrosine (ABA-tyr) in Lewis Rats: II. Induction and Suppression of ABA-Specific Helper Cell Activity for Antibody Production
| Autor: | Sidney Leskowitz, Nobuyuki Miyagawa, Chieh Yuan Lawn |
|---|---|
| Rok vydání: | 1982 |
| Předmět: |
medicine.medical_treatment
Immunology Intraperitoneal injection chemical and pharmacologic phenomena Spleen Biology complex mixtures Microbiology Adjuvants Immunologic p-Azobenzenearsonate Antigen Virology medicine Animals Lymphocytes Bovine serum albumin Immunity Cellular organic chemicals fungi food and beverages T-Lymphocytes Helper-Inducer Molecular biology Rats medicine.anatomical_structure Immunoglobulin M Rats Inbred Lew biology.protein Tyrosine Female Lymph Nodes Lymph Azo Compounds Haptens Hapten Adjuvant Keyhole limpet hemocyanin |
| Zdroj: | Microbiology and Immunology. 26:853-869 |
| ISSN: | 0385-5600 |
| Popis: | Immunization of Lewis rats with azobenzenearsonate-N-acetyl-L-tyrosine (ABA-tyr) in complete Freund's adjuvant (CFA), produces a hapten-specific helper T cell response measured by an increase in plaque forming cells (PFC) against a different hapten. The response seen is primarily direct (IgM) PFC unless B cells are primed by injection of trinitrophenylated keyhole limpet hemocyanin (TNP-KLH) prior to immunization with ABA-tyr. The response requires both ABA and TNP to be on the same carrier molecule which can be as diverse as bovine serum albumin (BSA), poly L-glutamine-lysine-tyrosine (L-GLT); however, a D-amino acid polypeptide does not work. The in vitro demonstration of such help was successful only with peritoneal exudate lymphocytes, not spleen or lymph node cells. Repeated pretreatment of rats by intraperitoneal injection of ABA-tyr in incomplete Freund's adjuvant (IFA) induced an unresponsiveness for helper activity to subsequent immunization with the same antigen in CFA. Passive transfer of lymphoid cells from spleens and lymph nodes from rats pretreated with ABA-tyr in IFA followed by boosting with ABA-tyr in CFA induced unresponsiveness to subsequent induction of hapten-specific help. |
| Databáze: | OpenAIRE |
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