Fluorescent detection of α-aminoadipic and γ-glutamic semialdehydes in oxidized proteins
| Autor: | Mitsugu Akagawa, Koji Uchida, Kyozo Suyama |
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| Rok vydání: | 2009 |
| Předmět: |
Adipates
Glutamic Acid Borohydrides Oxidative phosphorylation Sensitivity and Specificity Biochemistry Reductive amination Aldehyde High-performance liquid chromatography chemistry.chemical_compound Glutamates Physiology (medical) Chromatography High Pressure Liquid Fluorescent Dyes chemistry.chemical_classification Reactive oxygen species Chromatography Sodium cyanoborohydride Amino acid chemistry Reagent Reactive Oxygen Species 2-Aminoadipic Acid 4-Aminobenzoic Acid Oxidation-Reduction Protein Processing Post-Translational Protein Binding |
| Zdroj: | Free Radical Biology and Medicine. 46:701-706 |
| ISSN: | 0891-5849 |
| DOI: | 10.1016/j.freeradbiomed.2008.12.014 |
| Popis: | The oxidative modification of proteins is believed to play a critical role in the etiology and/or progression of several diseases. alpha-Aminoadipic semialdehyde (AAS) and gamma-glutamic semialdehyde (GGS) residues represent major oxidized amino acids generated in oxidized proteins. This paper describes a novel procedure for the specific and sensitive determination of AAS and GGS after their reductive amination with sodium cyanoborohydride and p-aminobenzoic acid, a fluorescence reagent, to their corresponding derivatives, followed by a high-performance liquid chromatography (HPLC) analysis. This fluorescent labeling of protein-associated aldehyde moieties is a simple and accurate technique that may be widely used to reveal increased levels of oxidatively modified proteins with reactive oxygen species during aging and disease. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
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