Svct2 expression and function in reactive astrocytes is a common event in different brain pathologies
| Autor: | Antonio J. López-Gambero, Laura Trigueros, Ernesto R. Bongarzone, Margarita Pérez-Martín, Francisca Espinoza, Manuel Cifuentes, María José Oviedo, Francisco Nualart, Luciano Ferrada, Katterine Salazar, Romina Bertinat, Katherine Forman, Natalia Saldivia, Christian Bonansco, Fernando Martínez |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2018 |
| Předmět: |
0301 basic medicine
Pathology medicine.medical_specialty Green Fluorescent Proteins Central nervous system Neuroscience (miscellaneous) Neuraminidase Hippocampus Biology Adenoviridae Rats Sprague-Dawley 03 medical and health sciences Cellular and Molecular Neuroscience 0302 clinical medicine Alzheimer Disease In vivo medicine Animals Gliosis Sodium-Coupled Vitamin C Transporters Neuroinflammation Brain medicine.disease Ascorbic acid Astrogliosis 030104 developmental biology medicine.anatomical_structure Neurology Cerebral cortex Astrocytes Kindling model 030217 neurology & neurosurgery |
| Zdroj: | Molecular Neurobiology Artículos CONICYT CONICYT Chile instacron:CONICYT |
| Popis: | Ascorbic acid (AA), the reduced form of vitamin C, acts as a neuroprotector by eliminating free radicals in the brain. Sodium/vitamin C co-transporter isoform 2 (SVCT2) mediates uptake of AA by neurons. It has been reported that SVCT2 mRNA is induced in astrocytes under ischemic damage, suggesting that its expression is enhanced in pathological conditions. However, it remains to be established if SVCT expression is altered in the presence of reactive astrogliosis generated by different brain pathologies. In the present work, we demonstrate that SVCT2 expression is increased in astrocytes present at sites of neuroinflammation induced by intracerebroventricular injection of a GFP-adenovirus or the microbial enzyme, neuraminidase. A similar result was observed at 5 and 10 days after damage in a model of traumatic injury and in the hippocampus and cerebral cortex in the in vivo kindling model of epilepsy. Furthermore, we defined that cortical astrocytes maintained in culture for long periods acquire markers of reactive gliosis and express SVCT2, in a similar way as previously observed in situ. Finally, by means of second harmonic generation and 2-photon fluorescence imaging, we analyzed brain necropsied material from patients with Alzheimer's disease (AD), which presented with an accumulation of amyloid plaques. Strikingly, although AD is characterized by focalized astrogliosis surrounding amyloid plaques, SVCT2 expression at the astroglial level was not detected. We conclude that SVCT2 is heterogeneously induced in reactive astrogliosis generated in different pathologies affecting the central nervous system (CNS). |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|