Decreased anchoring-fibril antigens (AF1 and AF2) in basal-cell carcinoma

Autor: J. E. Muhlbauer, Lowell A. Goldsmith, Patricia E. McCoon, Alfred T. Lane
Rok vydání: 1985
Předmět:
Zdroj: Archives of Dermatological Research. 277:499-501
ISSN: 1432-069X
0340-3696
DOI: 10.1007/bf00510070
Popis: Basement-membrane-zone (BMZ) components are altered in basal-cell carcinoma (BCC). Electron microscopy has identified areas of normal, discontinuous, or absent BMZ components in human BCC [13, 15, 26]. In the present study, we used new monoclonal antibodies to the anchoring fibrils to study this component of the BMZ. Skin tumors of 11 patients with BCC (10 nodular, 1 superficial) and the palmar pit of a patient with basal-cell nevus syndrome were examined [10] for bullous-pemphigoid antigen (BPA), laminin, and anchoring-fibril antigens recognized by monoclonal antibodies AF1 and AF2 [4, 5]. A wedge of tumor (1 2 mm) was frozen in optimal cutting temperature (OCT) compound (Lab-Tek Products, Naperville, IL, USA) and stored at -70~ until assayed. Fourmicron-thick sections were cut using a Tissue-Tek-II cryomicrotome and placed on gelatin-prepared slides. The tissue sections were air-dried for 20 rain before staining. AF1 and AF2 murine monoclonal antibodies to anchoring fibrils [4, 5] were used as undiluted hybridoma-cell supernatants. Human bullous-pemphigoid serum from a patient in whom the diagnosis had been confirmed by direct and indirect immunofluorescence
Databáze: OpenAIRE
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