Cellular, and subcellular localization of the mGluR5a metabotropic glutamate receptor in rat spinal cord
| Autor: | Tamás J. Görcs, Doris Rüegg, J. Hámori, Zoltán Vidnyánszky, László Négyessy, Thomas Knöpfel, Rainer Kuhn |
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| Rok vydání: | 1994 |
| Předmět: |
Male
Blotting Western Molecular Sequence Data Central nervous system Biology Neurotransmission Receptors Metabotropic Glutamate Postsynaptic potential medicine Animals Tissue Distribution Amino Acid Sequence Rats Wistar Neurons General Neuroscience Glutamate receptor Subcellular localization Spinal cord Immunohistochemistry Rats Cell biology Microscopy Electron Nociception medicine.anatomical_structure Spinal Cord nervous system Metabotropic glutamate receptor Neuroscience |
| Zdroj: | Scopus-Elsevier |
| ISSN: | 0959-4965 |
| DOI: | 10.1097/00001756-199412300-00053 |
| Popis: | The cellular and subcellular distribution of the mGluR5a metabotropic glutamate receptor was studied in the spinal cord of the rat using an antibody raised against a mGluR5a-specific carboxy-terminal peptide. Strong mGluR5a-immunoreactivity (mGluR5a-ir) was found in the laminae I-II of the dorsal horn, which gradually decreased towards the deeper layers. At the electron microscopical level, mGluR5a-ir was present exclusively in neuronal somata and dendrites. Immunometal labelling revealed that mGluR5a-ir is concentrated at the periphery of postsynaptic densities of asymmetrical synapses or localized extrasynaptically at dendritic and somatic membranes. The mGluR5a-immunoreactive dendritic profiles were often targeted by synaptic boutons with the morphological characteristics of C-fibre terminals. These observations provide evidence for mGluR5a being involved in the nociceptive transmission at the dorsal horn. |
| Databáze: | OpenAIRE |
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