Characteristics of a human epidermal squamous carcinoma cell line at different extracellular calcium concentrations
| Autor: | Joanne Miller, Glenn K. Walker, Miriam M. Brysk |
|---|---|
| Rok vydání: | 1984 |
| Předmět: |
Skin Neoplasms
Cell division Cell Biology Cell Line medicine Extracellular Humans Glycoproteins chemistry.chemical_classification Membrane Proteins Cell Biology Desmosomes Staining Cell biology Squamous carcinoma Culture Media Neoplasm Proteins medicine.anatomical_structure Membrane protein chemistry Cell culture Carcinoma Squamous Cell Calcium Epidermis Glycoprotein Cell Division |
| Zdroj: | Experimental cell research. 150(2) |
| ISSN: | 0014-4827 |
| Popis: | A continuous line derived from a human skin squamous cell carcinoma has been grown in media of high, normal and low Ca2+ concentrations. The growth rate was unaffected by the Ca2+ levels even though morphological changes were observed. Desmosomes were absent at low Ca2+ and areas of cell piling were observed at high Ca2+. Cell protein staining patterns on polyacrylamide gels were identical for cells grown at the three Ca2+ levels. The variations were minor for the glycoproteins reacted with 125I-conA. Lactoperoxidase iodination revealed changes in cell surface proteins, most markedly in the emergence of new proteins at high Ca2+. |
| Databáze: | OpenAIRE |
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