Electrospun degradable polyesterurethane membranes: potential scaffolds for skeletal muscle tissue engineering
| Autor: | Sara Mantero, Giulio Cossu, S.A. Riboldi, Maurilio Sampaolesi, Peter Neuenschwander |
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| Rok vydání: | 2004 |
| Předmět: |
Scaffold
Urethane Invitro MYOTUBE Myoblasts Tissue engineering Myosin satellite cell Myocyte Cell Differentiation block polyesterurethane Fibers Membrane medicine.anatomical_structure Mechanics of Materials Differentiation Biocompatibility BLOCK POLYESTERURETHANE ELECTROSPINNING SKELETAL MUSCLE TISSUE ENGINEERING SATELLITE CELL MYOBLAST C2C12 Muscle tissue Materials science Cell Survival Cells Polyesters Biophysics Bioengineering Cell Line Biomaterials medicine Cell Adhesion Humans Muscle Skeletal skeletal muscle tissue engineering electrospinning Tissue Engineering Membranes Artificial In-Vitro Ceramics and Composites Microscopy Electron Scanning myotube myoblast Therapy Principles Biomedical engineering |
| Zdroj: | Europe PubMed Central |
| ISSN: | 0142-9612 |
| Popis: | Skeletal muscle tissue engineering represents an attractive approach to overcome problems associated with autologous transfer of muscle tissue and provides a valid alternative in muscle regeneration enhancement. The aim of this study was to investigate the suitability, as scaffold for skeletal muscle tissue engineering, of a known biodegradable block copolymer (DegraPol (R)) processed by electrospinning in the novel form of microfibrous membranes. Scaffolds were characterized with reference to their morphological, degradative and mechanical properties. Subsequently, cell viability, adhesion and differentiation on coated and uncoated DegraPol (R) slides were investigated using line cells (C2C12 and L6) and primary human satellite cells (HSCs). The membranes exhibited absence of toxic residuals and satisfactory mechanical properties (linear elastic behavior up to 10% deformation, E modulus in the order of magnitude of MPa). A promising cellular response was also found in preliminary experiments: both line cells and HSCs adhered, proliferated and fused on differently coated electrospun membranes. Positive staining for myosin heavy chain expression indicated that differentiation of C2C12 multinucleated cells occurred within the porous elastomeric substrate. Together the results of this study provide significant evidence of the suitability of electrospun DegraPol (R) membranes as scaffolds for skeletal muscle tissue engineering and that they represent a promising alternative to scaffolds currently used in this field. (c) 2004 Elsevier Ltd. All rights reserved. ispartof: Biomaterials vol:26 issue:22 pages:4606-4615 ispartof: location:Netherlands status: published |
| Databáze: | OpenAIRE |
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