Identification of Major Locus Bph35 Resistance to Brown Planthopper in Rice
| Autor: | Li ZhenJing, Huang DaHui, Liu Fang, Li RongBai, MA Qianqian, Liu Chi, Qin Gang, Yang Xinghai, Wei Minyi, Liang Haifu, Zhang YueXiong, Ma ZengFeng |
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| Rok vydání: | 2020 |
| Předmět: |
0106 biological sciences
0301 basic medicine Genetics Candidate gene Bulked segregant analysis food and beverages Single-nucleotide polymorphism Locus (genetics) Plant Science lcsh:Plant culture Biology biology.organism_classification 01 natural sciences Oryza rufipogon 03 medical and health sciences 030104 developmental biology Chromosome 4 lcsh:SB1-1110 Brown planthopper Indel Agronomy and Crop Science 010606 plant biology & botany Biotechnology |
| Zdroj: | Rice Science, Vol 27, Iss 3, Pp 237-245 (2020) |
| ISSN: | 1672-6308 |
| DOI: | 10.1016/j.rsci.2020.04.006 |
| Popis: | An introgression line RBPH660, derived from wild rice Oryza rufipogon, showed stable resistance to brown planthopper (BPH). Segregation analysis indicated BPH resistance of RBPH660 was controlled by multiple genes/QTLs. By using the bulked segregant analysis (BSA)-seq method, two genomic regions harboring QTLs resistance to BPH were identified from 1.20 to 16.70 Mb on chromosome 4 and from 10.20 to 12.60 Mb on chromosome 9 in RBPH660, respectively. A major resistance locus, designated as Bph35 accounting for 51.27% of the phenotypic variation with a LOD score of 42.51, was mapped to the candidate region of chromosome 4 between InDel (insertion-deletion) markers PSM16 and R4M13. For fine mapping of Bph35, one simple sequence repeat and three newly developed InDel markers were used to screen the recombinants. Finally, the Bph35 locus was delimited in the region from 6.28 to 6.93 Mb and there were 18 predicted protein-encoding genes with a total of 114 non-synonymous single nucleotide polymorphism (SNP) variant sites between the resistant and susceptible parents. Out of these genes, Os04g0193950, encoding a putative NB-ARC (nucleotide- binding adaptor shared by APAF-1, R proteins and CED-4) and LRR (leucine-rich repeat) domain protein with nine non-synonymous SNP substitutions in its coding sequence regions, might be the candidate gene for Bph35. These findings would facilitate the map-based cloning of the Bph35 gene and development of resistant varieties against BPH in rice. Keywords: rice, brown planthopper, Bph35, bulked segregant analysis (BSA)-seq method, gene mapping |
| Databáze: | OpenAIRE |
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