Gamma-cross-linked nonfibrillar collagen gel as a scaffold for osteogenic differentiation of mesenchymal stem cells
| Autor: | Motohiro Hirose, Masahiko Bessho, Hajime Ohgushi, Hideki Mori, Masayuki Hara, Takako Takitoh |
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| Rok vydání: | 2015 |
| Předmět: |
Male
Scaffold Fibrillar Collagens Osteocalcin chemistry.chemical_element Bioengineering Calcium Applied Microbiology and Biotechnology Bone and Bones Dexamethasone Osteogenesis Cell Adhesion medicine Animals Cell Shape Cells Cultured Osteoblasts Tissue Engineering Tissue Scaffolds biology Mesenchymal stem cell Mesenchymal Stem Cells Osteoblast Anatomy Non-Fibrillar Collagens Rats Cell biology Collagen gel Collagen type I alpha 1 medicine.anatomical_structure chemistry Gamma Rays biology.protein Gels Biotechnology Gamma irradiation |
| Zdroj: | Journal of Bioscience and Bioengineering. 119:217-225 |
| ISSN: | 1389-1723 |
| DOI: | 10.1016/j.jbiosc.2014.07.008 |
| Popis: | We fabricated a transparent nonfibrillar collagen gel using gamma irradiation (5 kGy) and cultured rat mesenchymal stem cells (MSCs) on both the gamma-irradiated collagen gel and on unirradiated fibrillar collagen gel. Cells attached well and proliferated with high viability on the surface of both gels. The cells cultured on the gamma-irradiated nonfibrillar gel had a unique elongated shape and adhered to each other in culture. After 21 days of culture in dexamethasone-containing culture medium, the contents of bone-specific osteocalcin and calcium on the gamma-irradiated nonfibrillar gel were 1.4 and 1.9 times higher than those on fibrillar collagen gel, respectively. These data show that osteogenic differentiation of MSCs was promoted more efficiently on the gamma-cross-linked nonfibrillar gel than on the fibrillar gel and demonstrate the potential of the gamma-irradiated collagen gel for use in bone tissue engineering. |
| Databáze: | OpenAIRE |
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