The mucosal adjuvant effect of plant polysaccharides for induction of protective immunity against Helicobacter pylori infection
| Autor: | Da-Qun Chen, Chang Liu, Haibo Li, Cong-Jia Ma, Lu Ji, Quanming Zou, Kuan Miao, Ruo-Yi Xue, Ling Guo, Li Nie, Jiao Luo, Sun Li |
|---|---|
| Rok vydání: | 2018 |
| Předmět: |
Saliva
medicine.medical_treatment 030231 tropical medicine Helicobacter Infections 03 medical and health sciences Mice 0302 clinical medicine Immune system Antigen Adjuvants Immunologic Polysaccharides medicine Animals 030212 general & internal medicine Administration Intranasal Mice Inbred BALB C Mucous Membrane General Veterinary General Immunology and Microbiology biology Helicobacter pylori business.industry Vaccination Public Health Environmental and Occupational Health Th1 Cells biology.organism_classification Urease Immunoglobulin A TLR2 Ovalbumin Infectious Diseases Immunoglobulin G Immunology biology.protein Molecular Medicine Th17 Cells Female Immunization business Adjuvant |
| Zdroj: | Vaccine. 37(8) |
| ISSN: | 1873-2518 |
| Popis: | Some plant polysaccharides (PPSs) had been used as the adjuvants for systemic vaccination. In this study, we investigated whether PPSs could exhibit adjuvant effect at the mucosa. Groups of mice were intranasally immunized with Epimedium Polysaccharide (EPS), Trollius chinensis polysaccharide (TCPS), Siberian solomonseal rhizome polysaccharide (SSRPS) and Astragalus polysaccharides (APS) together with ovalbumin (OVA). Significantly higher levels of OVA-specific IgG in serum and secretory IgA in saliva, vaginal wash and intestinal lavage fluid were induced after immunization with OVA plus one of the four PPSs compared to OVA alone. Antigen absorption and TLR2 (Toll-like receptor 2) activation may be related to their mucosal adjuvant effect. Of note, when APS used as an adjuvant, intranasally vaccination with recombination UreB (rUreB, Urease subunit B) conferred more robust protection against Helicobacter pylori (H. pylori). Immunized with rUreB in combination APS resulted in mixed specific Th1 and Th17 immune response, which may contribute to the inhibition of H. pylori colonization. Though specific Th2-dominant responses were elicited when the other three PPS intranasally immunized with rUreB, no significant difference in the protective effect were found between those groups and rUreb alone group. Taken together, the four PPSs may be promising candidates for mucosal adjuvant, and APS could enhance rUreB-specific protective immunity against H. pylori infection. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|
Full Text from ScienceDirect