Proline Exogenously Supplied or Endogenously Overproduced Induces Different Nutritional, Metabolic, and Antioxidative Responses in Transgenic Tobacco Exposed to Cadmium
| Autor: | Thiago G. Ramires, Ricardo Antunes Azevedo, Carlos Alberto Labate, Flávio Henrique Silveira Rabêlo, Ilara Gabriela Frasson Budzinski, Patricia Dayane Carvalho Schaker, Lucélia Borgo, Alessandra Ferreira Ribas, José Lavres, Thais Regiani Cataldi, Ann Cuypers |
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| Přispěvatelé: | Universidade de São Paulo (USP), Hasselt University, Universidade Estadual Paulista (UNESP), Federal University of Technology of Paraná, University of Oeste Paulista, Escola Superior de Agricultura 'Luiz de Queiroz' |
| Jazyk: | angličtina |
| Rok vydání: | 2021 |
| Předmět: |
Nicotiana tabacum
Plant Science medicine.disease_cause Superoxide dismutase chemistry.chemical_compound Metabolite profiling medicine Proline Nicotiana tabacum L biology fungi Wild type food and beverages Plant physiology Glutathione biology.organism_classification chemistry Biochemistry Catalase Oxidative stress biology.protein Nutrient homeostasis P5CSF129A Agronomy and Crop Science |
| Zdroj: | Scopus Repositório Institucional da UNESP Universidade Estadual Paulista (UNESP) instacron:UNESP |
| Popis: | Made available in DSpace on 2022-04-28T19:44:09Z (GMT). No. of bitstreams: 0 Previous issue date: 2021-01-01 Proline plays adaptive roles in plant tolerance to cadmium (Cd)-induced stress, but many gaps remain to be elucidated as the responses triggered by exogenously supplied proline or endogenously overproduction are not well known. Thus, we assayed the nutritional status, metabolite profiling, and antioxidative responses in wild type and transgenic tobacco (Nicotiana tabacum L.) containing the P5CSF129A gene under control of the cauliflower mosaic virus (CaMV35S) or stress inducible rd29A promoters. The plants were exposed or unexposed to Cd (0 and 50 μmol L−1 CdCl2·H2O) for 24 and 72 h. The wild type plants were also treated with or without exogenous proline (1 mmol L−1). Plants supplied with exogenous proline exhibited lower Cd translocation from roots to leaves than plants overproducing proline, avoiding oxidative damages in the leaves of these plants. Meanwhile, tobacco overproducing proline was less susceptible to Cd-induced nutritional changes than wild type plants and presented better metabolic adjustment under Cd exposure compared to plants supplied with exogenous proline. Plants overproducing proline increased the synthesis of sugars and organic acids under Cd exposure, which contributed to absence of oxidative stress, since both superoxide dismutase and catalase were not active against Cd-induced oxidative stress in these genotypes. Plants overproducing proline under the control of rd29A presented higher proline concentration in comparison to the CaMV35S promoter. With exception of rd29A plants that presented high proline and reduced glutathione (GSH) concentrations, the other plants presented an inverse correlation between proline and GSH synthesis after 72 h of Cd exposure. College of Agriculture Luiz de Queiroz University of São Paulo Center for Nuclear Energy in Agriculture University of São Paulo Centre for Environmental Sciences Hasselt University Institute of Biosciences São Paulo State University Department of Mathematics Federal University of Technology of Paraná Federal University of Technology of Paraná Department of Agronomy University of Oeste Paulista Laboratório de Genética Bioquímica de Plantas Departamento de Genética Escola Superior de Agricultura “Luiz de Queiroz”, Avenida Pádua Dias, 11, Caixa Postal 9 Institute of Biosciences São Paulo State University |
| Databáze: | OpenAIRE |
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