Late-gestation maternal dietary methyl donor and cofactor supplementation in sheep partially reverses protection against allergic sensitization by IUGR
| Autor: | Gary K. Heinemann, Hong Liu, Joshua D. Rabinowitz, Vicki L. Clifton, Damien S. Hunter, Amy L. Wooldridge, Rebecca A. Simmons, Karen L. Kind, Yu-Chin Lien, Kathryn L. Gatford, Julie A. Owens, Robert J Bischof, Lynne C. Giles, Wenyun Lu |
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| Přispěvatelé: | Wooldridge, Amy L, Bischof, Robert J, Liu, Hong, Heinemann, Gary K, Hunter, Damien S, Giles, Lynne C, Simmons, Rebecca A, Lien, Yu-Chin, Lu, Wenyun, Rabinowitz, Joshua D, Kind, Karen L, Owens, Julie A, Clifton, Vicki L, Gatford, Kathryn L |
| Rok vydání: | 2018 |
| Předmět: |
0301 basic medicine
Physiology Placenta Intrauterine growth restriction mast cells Dermatitis Immunoglobulin E Allergic sensitization Methionine 0302 clinical medicine Pregnancy Mast Cells Skin Fetal Growth Retardation Pyroglyphidae Gestational age Cobalt animal models medicine.anatomical_structure In utero Prenatal Exposure Delayed Effects Female Research Article medicine.medical_specialty intrauterine growth restriction Ovalbumin Gestational Age Biology folic acid 03 medical and health sciences Folic Acid Physiology (medical) Internal medicine Hypersensitivity medicine Animals Sheep Domestic House dust mite DNA Methylation medicine.disease biology.organism_classification methyl donors Disease Models Animal 030104 developmental biology Endocrinology Dietary Supplements biology.protein fetal growth Sulfur 030215 immunology |
| Zdroj: | American Journal of Physiology-Regulatory, Integrative and Comparative Physiology. 314:R22-R33 |
| ISSN: | 1522-1490 0363-6119 |
| Popis: | Perinatal exposures are associated with altered risks of childhood allergy. Human studies and our previous work suggest that restricted growth in utero (IUGR) is protective against allergic disease. The mechanisms are not clearly defined, but reduced fetal abundance and altered metabolism of methyl donors are hypothesized as possible underlying mechanisms. Therefore, we examined whether late-gestation maternal dietary methyl donor and cofactor supplementation of the placentally restricted (PR) sheep pregnancy would reverse allergic protection in progeny. Allergic outcomes were compared between progeny from control pregnancies (CON; n = 49), from PR pregnancies without intervention (PR; n = 28), and from PR pregnancies where the dam was fed a methyl donor plus cofactor supplement from day 120 of pregnancy until delivery (PR + Methyl; n = 25). Both PR and PR + Methyl progeny were smaller than CON; supplementation did not alter birth size. PR was protective against cutaneous hypersensitivity responses to ovalbumin (OVA; P < 0.01 in singletons). Cutaneous hypersensitivity responses to OVA in PR + Methyl progeny were intermediate to and not different from the responses of CON and PR sheep. Cutaneous hypersensitivity responses to house dust mites did not differ between treatments. In singleton progeny, upper dermal mast cell density was greater in PR + Methyl than in PR or CON (each P < 0.05). The differences in the cutaneous allergic response were not explained by treatment effects on circulating immune cells or antibodies. Our results suggest that mechanisms underlying in utero programming of allergic susceptibility by IUGR and methyl donor availability may differ and imply that late-gestation methyl donor supplementation may increase allergy risk. |
| Databáze: | OpenAIRE |
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