Coordinated regulation of the dorsal-ventral and anterior-posterior patterning ofXenopusembryos by the BTB/POZ zinc finger protein Zbtb14

Autor:	Misa Uchida, Atsushi Suzuki, Kimiko Takebayashi-Suzuki, Hidenori Konishi, Tomoko Nagata, Tatsuo Miyamoto
Rok vydání:	2018
Předmět:	Smad5 Protein 0301 basic medicine Embryo Nonmammalian Kruppel-Like Transcription Factors Xenopus Xenopus Proteins Bone morphogenetic protein Fibroblast growth factor Smad1 Protein Xenopus laevis 03 medical and health sciences Animals Body Patterning Zinc finger biology Embryogenesis Wnt signaling pathway Gene Expression Regulation Developmental Zinc Fingers Cell Biology biology.organism_classification Cell biology Fibroblast Growth Factors 030104 developmental biology Smad8 Protein Neural development Developmental Biology Transforming growth factor
Zdroj:	Development, Growth & Differentiation. 60:158-173
ISSN:	0012-1592
DOI:	10.1111/dgd.12431
Popis:	During early vertebrate embryogenesis, bone morphogenetic proteins (BMPs) belonging to the transforming growth factor-β (TGF-β) family of growth factors play a central role in dorsal-ventral (DV) patterning of embryos, while other growth factors such as Wnt and fibroblast growth factor (FGF) family members regulate formation of the anterior-posterior (AP) axis. Although the establishment of body plan is thought to require coordinated formation of the DV and AP axes, the mechanistic details underlying this coordination are not well understood. Here, we show that a Xenopus homologue of zbtb14 plays an essential role in the regulation of both DV and AP patterning during early Xenopus development. We show that overexpression of Zbtb14 promotes neural induction and inhibits epidermal differentiation, thereby regulating DV patterning. In addition, Zbtb14 promotes the formation of posterior neural tissue and suppresses anterior neural development. Consistent with this, knock-down experiments show that Zbtb14 is required for neural development, especially for the formation of posterior neural tissues. Mechanistically, Zbtb14 reduces the levels of phosphorylated Smad1/5/8 to suppress BMP signaling and induces an accumulation of β-Catenin to promote Wnt signaling. Collectively, these results suggest that Zbtb14 plays a crucial role in the formation of DV and AP axes by regulating both the BMP and Wnt signaling pathways during early Xenopus embryogenesis.
Databáze:	OpenAIRE
Externí odkaz:	https://explore.openaire.eu/search/publication?articleId=doi_dedup___::0f5fd9d532f0be6b2582f7e0cc1ef6a7 https://doi.org/10.1111/dgd.12431 Zobrazit plný text záznamu Plný text ve formátu PDF Plný text ve formátu HTML
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