Loss of the small GTPase Arl8b results in abnormal development of the roof plate in mouse embryos
| Autor: | Yoshifumi Yamaguchi, Masayuki Miura, Keisuke Hashimoto, Kanako Takasaki, Shinya Ohata, Yorifumi Kikko, Yusuke Kishi, Yurie Maeda, Miho Oka, Toshiaki Katada, Yudai Matsumoto, Kenji Kontani |
|---|---|
| Rok vydání: | 2018 |
| Předmět: |
Neural Tube
animal structures SMAD Biology 03 medical and health sciences Mice SOX1 Genetics medicine Animals 030304 developmental biology Monomeric GTP-Binding Proteins 0303 health sciences Neural fold ADP-Ribosylation Factors SOXB1 Transcription Factors Embryogenesis Neural tube Gene Expression Regulation Developmental Cell Biology Cell biology Neuroepithelial cell Mice Inbred C57BL medicine.anatomical_structure Neural Crest GDF7 embryonic structures Ectopic expression Lysosomes Signal Transduction |
| Zdroj: | Genes to cells : devoted to molecularcellular mechanisms. 24(6) |
| ISSN: | 1365-2443 |
| Popis: | Lysosomes are acidic organelles responsible for degrading both exogenous and endogenous materials. The small GTPase Arl8 localizes primarily to lysosomes and is involved in lysosomal function. In the present study, using Arl8b gene-trapped mutant (Arl8b-/- ) mice, we show that Arl8b is required for the development of dorsal structures of the neural tube, including the thalamus and hippocampus. In embryonic day (E) 10.5 Arl8b-/- embryos, Sox1 (a neuroepithelium marker) was ectopically expressed in the roof plate, whereas the expression of Gdf7 and Msx1 (roof plate markers) was reduced in the dorsal midline of the midbrain. Ectopic expression of Sox1 in Arl8b-/- embryos was detected also at E9.0 in the neural fold, which gives rise to the roof plate. In addition, the levels of Bmp receptor IA and phosphorylated Smad 1/5/8 (downstream of BMP signaling) were increased in the neural fold of E9.0 Arl8b-/- embryos. These results suggest that Arl8b is involved in the development of the neural fold and the subsequently formed roof plate, possibly via control of BMP signaling. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|
Plný text