Identification of phospholipid as an essential part of bovine vitamin K-dependent carboxylase

Autor:	H. Coenraad Hemker, Berry A.M. Soute, Cees Vermeer, Menno de Metz
Přispěvatelé:	Biochemie
Rok vydání:	1981
Předmět:	chemistry.chemical_classification Vitamin fungi Phospholipid food and beverages Cell Biology Phospholipase Biochemistry Micelle In vitro Pyruvate carboxylase chemistry.chemical_compound Enzyme chemistry Phosphatidylcholine Molecular Biology
Zdroj:	Journal of Biological Chemistry, 256(21), 10843-10846. American Society for Biochemistry and Molecular Biology, Inc.
ISSN:	0021-9258
DOI:	10.1016/s0021-9258(19)68520-0
Popis:	Vitamin K-dependent carboxylase from bovine liver contains phospholipid (primarily phosphatidylcholine), which is essential for its in vitro activity. Sepharose-bound carboxylase can be depleted of phospholipids, either by washing the enzyme with detergents or by phospholipase treatment. The enzyme can be reconstituted by adding mixed micelles of phosphatidylcholine and cholate to the Sepharose-bound proteins.
Databáze:	OpenAIRE
Externí odkaz:	https://explore.openaire.eu/search/publication?articleId=doi_dedup___::0f5cf3166d9c0f3109030ddefa2910f6 https://doi.org/10.1016/s0021-9258(19)68520-0 Zobrazit plný text záznamu