Alveolar but not intravenous S-ketamine inhibits alveolar sodium transport and lung fluid clearance in rats
| Autor: | Catharina W. Wieland, Stefanie Zügel, Bernhard Pitzer, Marc M. Berger, Albert Dahan, Heimo Mairbäurl, Markus W. Hollmann, Peter Bärtsch, Marcus J. Schultz, Alexander P.J. Vlaar |
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| Přispěvatelé: | Amsterdam institute for Infection and Immunity, Intensive Care Medicine, Amsterdam Cardiovascular Sciences, Anesthesiology |
| Jazyk: | angličtina |
| Rok vydání: | 2010 |
| Předmět: |
Lipopolysaccharides
Male Atmosphere Exposure Chambers medicine.medical_specialty Sodium chemistry.chemical_element Cell Separation Sodium Channels Rats Sprague-Dawley Sepsis Internal medicine medicine Animals Ketamine Lung Cells Cultured Anesthetics Dissociative business.industry fungi Antagonist Glutamate receptor Skeletal muscle respiratory system medicine.disease Endotoxemia Body Fluids Rats Pulmonary Alveoli Anesthesiology and Pain Medicine Endocrinology medicine.anatomical_structure chemistry Injections Intravenous NMDA receptor business Bronchoalveolar Lavage Fluid Sodium Channel Blockers medicine.drug |
| Zdroj: | Anesthesia and analgesia, 111(1), 164-170. Lippincott Williams and Wilkins |
| ISSN: | 0003-2999 |
| Popis: | BACKGROUND: S-ketamine is frequently used for analgosedation, especially during sepsis and cardiovascular instability. Because S-ketamine blocks voltage-gated sodium (Na+) channels in neurons and skeletal muscle, it is conceivable that S-ketamine also blocks alveolar epithelial Na+ channels that are crucial for alveolar fluid clearance (AFC). We studied the effects of alveolar and IV S-ketamine on transalveolar Na+ transport and AFC, and investigated whether IV S-ketamine enters the alveolar space in response to endotoxemia-induced pulmonary inflammation. METHODS: Cultured rat alveolar type II (ATII) cells were exposed to S-ketamine and/or the Na+ channel blocker amiloride (100 microM) and transepithelial transport indicated by short circuit current (ISC) was measured in Ussing chambers. AFC was measured in fluid-instilled lungs of anesthetized rats with or without amiloride added to the instillate. S-ketamine was either added to the instillate or injected IV. To induce mild lung injury that might favor the appearance of IV S-ketamine at the alveolar surface, endotoxemia was induced by IV lipopolysaccharide (7.5 mg/kg). RESULTS: In ATII cells, S-ketamine (25 microg/mL) caused a decrease of ISC regardless of apical (-18.9%+/- 1.4%; P |
| Databáze: | OpenAIRE |
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