Liver, but not adipose tissue PEDF gene expression is associated with insulin resistance
| Autor: | F Ortega, Gerard Pardo, Mònica Sabater, José Manuel Fernández-Real, J M Moreno-Navarrete, Zdeňka Lacinová, Wifredo Ricart, Marta Serrano, Victoria Catalán, Gema Frühbeck, J. Drápalová, Martin Haluzik, Milos Mraz, Javier Gómez-Ambrosi, Neus Pueyo, M. R. Ortiz, V Touskova |
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| Rok vydání: | 2012 |
| Předmět: |
Adult
Male medicine.medical_specialty Endocrinology Diabetes and Metabolism Medicine (miscellaneous) Adipose tissue Enzyme-Linked Immunosorbent Assay White adipose tissue Type 2 diabetes Biology Real-Time Polymerase Chain Reaction Body Mass Index chemistry.chemical_compound PEDF Insulin resistance Internal medicine Gene expression medicine Adipocytes Humans Longitudinal Studies Nerve Growth Factors Eye Proteins Cells Cultured Serpins Nutrition and Dietetics Cell Differentiation Hep G2 Cells medicine.disease Obesity Morbid Real-time polymerase chain reaction Endocrinology Cross-Sectional Studies chemistry Adipose Tissue Liver Spain Female Glycated hemoglobin Insulin Resistance |
| Zdroj: | International journal of obesity (2005). 37(9) |
| ISSN: | 1476-5497 |
| Popis: | Recent studies linked circulating pigment epithelium-derived factor (PEDF) to obesity-associated insulin resistance, but the main source of circulating PEDF is unknown. We aimed to investigate liver and adipose tissue PEDF gene expression in association with obesity and insulin resistance. Three (two cross-sectional and one longitudinal) independent cohorts have been studied, for adipose tissue (n=80 and n=30) and liver gene expression (n=32 and n=14). Effects of high glucose and cytokines on HepG2 cell line were also investigated. PEDF gene expression and circulating PEDF were analyzed using real-time PCR and ELISA, respectively. In a first cohort of subjects, PEDF relative gene expression was higher in subcutaneous (SC) than in omental (OM) adipose tissue (P |
| Databáze: | OpenAIRE |
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